Cell-based Assays

APOPercentage is a colorimetric, dye-based assay for detection and measurement of apoptosis (programmed cell death) during in-vitro cell culture.

Quantification of Reduced Glutamine - Easy extraction of cell Lysate - Used to determine intracellular levels of Glutamine

Quantification of Reduced Glutamate - Easy extraction of cell Lysate - Used to determine intracellular levels of Glutamate

Cell Proliferation / Cytotoxicity Assay Kit ∼ Feature ∼ 1.More Sensitive than MTT, MTS, or WST-1 2.No Toxicity to Cell 3.3 Simple Steps (No Thawing Necessary) 4.Stable One Bottle Solution : 1 year at 5℃

Cell Proliferation / Cytotoxicity Assay Kit 1.More Sensitive than MTT, MTS, or WST-1 2.No Toxicity to Cell 3.3 Simple Steps (No Thawing Necessary) 4.Stable One Bottle Solution : 1 year at 5℃

Cell Proliferation / Cytotoxicity Assay Kit ∼ Feature ∼ 1.More Sensitive than MTT, MTS, or WST-1 2.No Toxicity to Cell 3.3 Simple Steps (No Thawing Necessary) 4.Stable One Bottle Solution : 1 year at 5℃

Please note this product may be subject to fees, we invite you to contact your local office. The recombinant expression vectors are designed to express human engineered T cell receptor (TCR) activator and human PD-L1 (GenBank Accession #NM_014143) in mammalian cells. The transfected cells can be used in conjunction with PD-1/NFAT Reporter/Jurkat T cells (BPS #60535) to study the interactions of PD-1 with PD-L1 ligand in a cellular context and screen for modulators of this signaling pathway. In this assay, PD-1/NFAT Reporter/Jurkat T cells are used as effector cells; HEK293 cells overexpressing PD-L1 (or PD-L2) and an engineered T cell receptor (TCR) activator are used as target cells. When these two cells are co-cultivated, TCR complexes on effector cells are activated by TCR activator on target cells, resulting in expression of the NFAT luciferase reporter. However, PD1 and PD-L1 (or PD-L2) ligation prevents TCR activation and suppresses the NFAT-responsive luciferase activity. This

Please note this product may be subject to fees, we invite you to contact your local office. The recombinant expression vectors are designed to express human engineered T cell receptor (TCR) activator and human PD-L2 (GenBank Accession #NM_025239) in mammalian cells. The transfected cells can be used in conjunction with PD-1/NFAT Reporter/Jurkat T cells (BPS #60535) to study the interactions of PD-1 with PD-L2 ligand in a cellular context and screen for modulators of this signaling pathway. In this assay, PD-1/NFAT Reporter/Jurkat T cells are used as effector cells; HEK293 cells overexpressing PD-L1 (or PD-L2) and an engineered T cell receptor (TCR) activator are used as target cells. When these two cells are co-cultivated, TCR complexes on effector cells are activated by TCR activator on target cells, resulting in expression of the NFAT luciferase reporter. However, PD1 and PD-L1 or PD-L2 ligation prevents TCR activation and suppresses the NFAT-responsive luciferase activity. This in