Functional Assays

The VEGFR1:VEGF165 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA designed for screening and profiling molecules that block the binding between VEGFR1 (vascular endothelial growth factor receptor 1, also known as fms related receptor tyrosine kinase 1) and VEGF165 (vascular endothelial growth factor 165). This kit comes in a convenient 96-well format, with enough recombinant biotin-labeled VEGF165 (amino acids 27-191(end)), purified VEGFR1 (amino acids 27-756), streptavidin-labeled HRP, and assay buffer for 100 binding reactions. A 96-well plate is coated with VEGFR1 protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-VEGF165, the plate is washed and Streptavidin-HRP is added. The ELISA ECL substrate is added, and the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of VEGFR1 to VEGF165.

The Molecular Glue/PROTAC® Optimization Kit for CDK2/CDK9-Cereblon Binding is designed for the testing and profiling of Molecular Glues (MG) and PROTACs targeting CDK2 (cyclin dependent kinase 2) or CDK9 (cyclin dependent kinase 9) and Cereblon (CRBN). The Molecular Glue/PROTAC® Optimization Kit for CDK2/CDK9-Cereblon Binding comes in a convenient AlphaLISA® format, with enough PROTAC® buffer, purified CDK2/CyclinA2 and CDK9/CyclinK complexes, and CRBN for 384 reactions. This kit also contains the control PROTAC® (CDK2/9 Degrader) and CDK inhibitor FN-1501.The Molecular Glue/PROTAC® of interest is incubated with Cereblon (CRBN) and CDK2 or CDK9, bringing them into proximity. CRBN contains a FLAG-tag, which is recognized by anti-FLAG AlphaLISA„¢ acceptor bead. CDK contains a GST-tag that binds to the donor bead. Upon excitation of the donor bead, a singlet oxygen is generated by the bead. The singlet oxygen excites the acceptor bead, which emits light proportionally to the level of i