Functional Assays

Glycogen phosphorylase is divided into active glycogen phosphorylase a (Glycogen phosphorylase a, GPa) and inactive glycogen phosphorylase b (Glycogen phosphorylase b, GPb) two forms. The decomposition of glycogen is mainly carried out under the catalysis of glycogen phosphorylase a. CheKine™ Micro Glycogen Phosphorylase a (GPa) Activity Assay Kit can detect animal tissues, cells or bacteria, plasma, serum or other liquid samples. In this kit, when no activator is added, GPa catalyzes the production of glucose residues from glycogen and inorganic phosphorus to glycogen and glucose 1-phosphate. Under the action of phosphoglucose mutase and 6-phosphate glucose dehydrogenase, it further catalyzes the reduction of NADP to NADPH. Measuring the rate of increase of NADPH at 340 nm can reflect the activity of GPa.

Glycogen phosphorylase is divided into active glycogen phosphorylase a (Glycogen phosphorylase a, GPa) and inactive glycogen phosphorylase b (Glycogen phosphorylase b, GPb) two forms. The decomposition of glycogen is mainly carried out under the catalysis of glycogen phosphorylase a. CheKine™ Micro Glycogen Phosphorylase b (GPb) Activity Assay Kit can detect animal tissues, cells or bacteria, plasma, serum or other liquid samples. In this kit, when no activator is added, GPa catalyzes the production of glucose residues from glycogen and inorganic phosphorus to glycogen and glucose 1-phosphate. Under the action of phosphoglucose mutase and 6-phosphate glucose dehydrogenase, it further catalyzes the reduction of NADP to NADPH. Measuring the rate of increase of NADPH at 340 nm can reflect the activity of GPa. The activity of GP (GPa and GPb) is measured by adding an activator. GPb activity can be obtained by subtracting GPa activity from GP activity

UDP-glucose pyrophosphorylase (UGP, EC2.7.7.9) is widely distributed in nature. It catalyzes the activation of glucose before glycogen synthesis. UDP-glucose (UDPG) is synthesized from glucose-1-phosphate and UTP. UDPG is the main active enzyme form in higher plants and animals. As a glucose-based donor, it participates in the synthesis and metabolism of glycogen, sucrose, cellulose, etc. CheKine™ Micro UDP-Glucose Pyrophosphosphprylase (UGP) Activity Assay Kit can detect animal and plant tissues, cells, plasma, serum or other liquid samples. In this kit, UGP can catalyze the reversible formation of glucose-1-phosphate. NADP was transformed into NADPH by phosphoglucose mutase and 6-phosphoglucose dehydrogenase. UGP activity can be reflected by the change of 340 nm absorption value.

ADP-glucose pyrophosphorylase (AGP, EC2.7.7.21) mainly exists in plants, glucose-1-phosphoric acid catalytic reaction with ATP generation of starch synthesis precursor ADPG directly, is the main limiting step of plant starch biosynthesis. CheKine™ Micro ADP-Glucose Pyrophosphorylase (AGP) Activity Assay Kit can detect plant tissues. In this kit, the reverse reaction catalyzed by AGP generates G1P, and the hexose phosphate mutase and glucose 6-phosphate dehydrogenase added to the reaction system catalyze 6-phosphogluconic acid and NADPH in turn. The AGP activity can be calculated by measuring the increase rate of NADPH at 340 nm.