Functional Assays

ACP (Acid Phosphatase) catalyzes the hydrolysis of phosphomonoesters into inorganic phosphate under acidic conditions, commonly found within lysosomes of macrophages. ACP is frequently employed as an auxiliary diagnostic tool for prostate cancer. CheKine™ Micro Acid Phosphatase (ACP) Activity Assay Kit provides a simple, convenient, and rapid method for determining ACP activity, applicable to a variety of samples including plant and animal tissues, serum, and plasma. Its principle relies on the ACP-catalyzed hydrolysis of sodium phenyl phosphate to produce phenol in an acidic milieu; subsequently, phenol reacts with 4-aminoantipyrene and potassium ferricyanide to form a red quinone derivative which exhibits characteristic light absorption at 510 nm. By measuring the rate of increase in absorbance at 510 nm, the ACP activity can be calculated.

Chalcone isomerase (CHI) is the first recognized enzyme related to flavonoid synthesis and one of the key enzymes in flavonoid metabolism. Chalcone isomerase and chalcone synthase together constitute rate-limiting enzymes for flavonoid biosynthesis. CheKine™ Micro Chalcone Isomerase (CHI) Activity Assay Kit can detect plant tissues samples. In this kit, CHI catalyzed the cyclization of chalcone to form 4,5,7-trihydroxyflavanones, and the activity of CHI was indicated by measuring the absorbance change at 381 nm.

Malic enzyme (ME) is widely present in the cytoplasm of microorganisms, cultured cells, animals, and plants, especially with high activity in plant tissues. ME catalyzes the reversible oxidation decarboxylation of malic acid, producing pyruvate and CO2, as well as the reduction reaction accompanied by NAD(P)+, which is a key enzyme in malic acid metabolism. ME activity is closely related to biosynthesis and antioxidant activity. Based on the specificity of coenzyme and substrate, ME can be divided into NAD-ME (EC1.1.1.38) and NADP-ME (EC1.1.1.40). NAD-ME can catalyze the reduction of NAD+ to NADH, and the rate of NADH increase at 340 nm can reflect its activity.

SucrosePhosphorylase (SP, EC2.4.1.7) is mainly present in microorganisms and plants and belongs to the glycosylhydrolase 13 family. It is an enzyme that catalyzes the transfer of glucoside bonds and can catalyze the synthesis of 1-phospho-glucose from sucrose and inorganic phosphate. This enzyme mainly uses sucrose and glucose 1-phosphate as donors, and many substances such as polyhydroxyl sugars, sugar alcohols, phenolic hydroxyl groups, carboxyl groups as receptors to catalyze the synthesis of various glycosides. CheKine™ Micro Sucrose Phosphorylase (SP) Activity Assay Kit can detect plant tissues, fungus samples. In this kit, SP can catalyze sucrose to produce glucose 1-phosphate, which is modified to glucose 6-phosphate under the catalysis of glucose phosphomutase, and reduce NADP+ to generate NADPH under the action of glucose 6-phosphate dehydrogenase, resulting in an increase in the light absorption value of 340nm. The SP activity was reflected by the increase rate of 340nm abs

used to detect triglyceridel levels

used to detect cholestrol levels

used to detect Phospholipid levels

The METTL3/METTL14 Complex Chemiluminescent Assay Kit is designed to measure METTL3/METTL14 complex activity for screening and profiling applications. The key to the METTL3/METTL14 Complex Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes N6-methylated adenosine. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

The ADAR1:RNA TR-FRET Assay Kit is designed to measure the binding of ADAR1 (adenosine deaminase, RNA-specific 1) to RNA for screening and profiling applications using TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer). It utilizes Terbium-labeled donor and dye-labeled acceptor to complete the TR-FRET pairing. The ADAR1:RNA TR-FRET Assay Kit comes in a convenient 384-well format, with enough purified ADAR1, Tb-Labeled Donor and Dye-Labeled Acceptor, RNase inhibitor, ADAR1 substrate and assay buffer for 384 reactions. The assay also includes a Competitor RNA as control.