PEG

DSPE-PEG-Fluor 555, MW 2,000 is a PEG lipid conjugate with a DSPE group and a Fluor 555 dye. Fluor 555 is a bright orange cyanine-based dye while DSPE is a phospholipid frequently employed in the development of lipid nanoparticles.

t-Boc-N-Amido-PEG8-Ms is a heterobifunctional PEG linker featuring a Boc-protected amine and a mesylate group. Mesylates are a good leaving groups which are readily displaced by nucleophiles while the Boc protecting group can be removed with acid to reveal an amine which can be used in a variety of chemical transformations.

Mal-amido-PEG4-amine TFA salt is a short PEG linker featuring a maleimide and a primary amine. Maleimide is a thiol-specific covalent linker which is used to label cysteine residues on proteins, while primary amines can be used in a variety of ways such as reductive aminations or couplings.

Bis-sulfone Amine is a reagent featuring two sulfone groups and a primary amine. The sulfone group is a covalent ligand which is used to bind thiols via a Michael addition, and this can be employed to label cysteine residues in proteins. The presence of two leaving groups on this molecule allows this linker to bind two thiols at a time. The amine group is free to perform a variety of reactions such as coupling with carboxylic acids, reductive amination with aldehydes or ketones, or SNAr with electron-deficient aromatic groups.

8-Azido-3,8-dideoxy-D-manno-octulosonic acid (Kdo Azide) is an analogue of the natural 3-deoxy-D-manno-octulosonic acid (Kdo) that contains a very small modification, specifically an azido moiety. This molecule can be incorporated by cultured cells (Gram-negative Bacteria, for example) and incorporated into glycans (LPS, for example) during active glycan biosynthesis. Detection utilizes the chemoselective ligation or “click” reaction between an azide and an alkyne or cyclooctyne.

DBCO-PEG5-TFP Ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic polyethylene glycol (PEG) spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.

BP Fluor 594 Biotin is a chemical reagent containing the BP Fluor 594 dye and a biotin group. Biotin is an affinity ligand and it is used in biochemical applications such as pull-down assays or for ligating with streptavidin proteins. BP Fluor 594 is a red fluorescent dye with excitation and emission maxima at 590 nm and 617 nm respectively.

TCO-PEG4-TFP Ester is an amine-reactive labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. A 2,3,5,6-tetrafluorophenol (TFP) is a reactive ester that displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers. TFP ester of carboxylic acids react with primary amines at the same rate as NHS ester forming covalent amide bond that is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters.

Methyltetrazine-PEG4-STP Ester is an amine-reactive, water-soluble labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers in aqueous media. Methyltetrazine-PEG4-STP Ester undergoes hydrolysis in aqueous media at much slower rate compared to Methyltetrazine-Sulfo NHS Ester allowing for more efficient labeling of biomolecules in aqueous media.