PEG

Sulfo DBCO-TFP Ester is a water-soluble, amine-reactive labeling reagent that enables simple and efficient incorporation of Sulfo DBCO moiety onto amine-containing molecules. The hydrophilic, sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules, in many cases making them completely soluble in aqueous media. A short spacer arm adds minimal mass to modified molecules.

Alkyne Sphinganine a metabolic labeling reagent that contains a terminal alkyne moiety that allows for the subsequent tagging of protein−lipid complexes by click chemistry. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as ‘click chemistry’, in the presence of a copper (Cu)-containing catalyst allowing for the visualization and proteomic profiling of protein−sphingolipid complexes in living cells.

Metabolic labeling with unnatural sugar substrates is a powerful alternative for investigating glycoconjugates in living organisms. Trehalose glycolipids can be metabolically labeled with 6-azide-modified trehalose (TreAz) analogues in live mycobacteria, enabling bioorthogonal ligation with alkyne-functionalized fluorescent probes. This strategy can be used for imaging glycolipid distribution, trafficking, and dynamics as well as metabolite profiling and discovery.

Metabolic labeling with unnatural sugar substrates is a powerful alternative for investigating glycoconjugates in living organisms. Trehalose glycolipids can be metabolically labeled with O-Alkyne-Trehalose (O-AlkTMM) analogues in live mycobacteria, enabling bioorthogonal ligation with azide-functionalized fluorescent probes. This strategy can be used for imaging glycolipid distribution, trafficking, and dynamics as well as metabolite profiling and discovery.

Disulfide Biotin alkyne is an azide-activated cleavable biotin probe that allows for efficient recovery of avidin-bound protein complexes in affinity-based assays. This reagent contains a biotin moiety linked to an alkyne group through a spacer arm containing a cleavable disulfide linker. Under reducing conditions (50 mM dithiothreitol, 10 mM 2-mercaptoethanol or 1% sodium borohydride), the disulfide bonds are cleaved, releasing the biotin tag and any avidin conjugate bound to it.

DOPE-PEG-Fluor 488, MW 2,000 is a PEG lipid featuring a DOPE phospholipid and a Fluor 488 dye. DOPE is a phospholipid that is known to spontaneously form micelles in water and is featured heavily in lipid nanoparticles and drug development, while Fluor 488 is a cyanine dye that is prominently used in fluorescence microscopy. Fluor 488 has excitation and emission maxima at 499 nm and 520 nm.

Biotin-PEG3-Bromide is a short PEG linker featuring a biotin group and a bromide. The bromide is a halogen which is easily displaced by nucleophiles such as alcohols or amines. Alternatively, bromide can be applied in a number of cross-coupling reactions such as in a Suzuki reaction. Biotin is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins.

N-(Azide-PEG8)-N-bis(PEG8-Amine) is a trifunctional PEG reagent containing two primary amines and one azide. The primary amine is reactive with carboxylic acids, NHS esters, or carbonyls to form a variety of stable bonds. The azide can be used to do click chemistry with terminal alkynes or strained cyclooctynes like DBCO or BCN.

DOPE-PEG-Fluor 488, MW 5,000 is a PEG lipid featuring a DOPE phospholipid and a Fluor 488 dye. DOPE is a phospholipid that is known to spontaneously form micelles in water and is featured heavily in lipid nanoparticles and drug development, while Fluor 488 is a cyanine dye that is prominently used in fluorescence microscopy. Fluor 488 has excitation and emission maxima at 499 nm and 520 nm.