PEG

Biotin-PEG8-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azide groups on a target molecule. The inclusion of a PEG linker in this molecule improves its solubility in water.

TCO-PEG4-Val-Cit-PFP Ester is a cleavable ADC linker featuring a TCO group, a Val-Cit dipeptide, and a PFP ester. The TCO group is reactive towards tetrazine groups on target compounds in a high yielding click reaction. PFP is a good leaving group which is readily displaced by amines to form amides. Finally the Val-Cit dipeptide is easily cleaved by enzymes to allow for payload release within the cell.

DBCO-N-bis(PEG8-acid) is a trifunctional molecule containing two carboxylic acids and a DBCO functional group. The DBCO is reactive towards azides in copper-free click chemistry while the carboxylic acids can be reacted with primary amines in the presence of a coupling reagent to form stable amide bonds. PEG linkers have higher aqueous solubility than their alkane counterparts, which may impact DMPK and ADME in vivo.

DBCO-N-bis(PEG8-NHS ester) is a trifunctional reagent containing two activated NHS esters and a DBCO moiety. The DBCO is a strained cyclooctyne and reacts spontaneously with azide groups to form stable triazole links with the target molecule. The NHS esters are activated towards attack from nucleophiles such as amines and alcohols to form amides or esters respectively.

N-DBCO-N-bis(PEG4-t-butyl ester) is a trifunctional chemical containing two t-butyl esters and a DBCO functional group. The DBCO is reactive towards azides in copper-free click chemistry while the t-butyl esters can be deprotected and used in coupling with amines or alcohols to form amides or esters respectively.

NH-bis(PEG8-Acid), HCl salt is a bifunctional reagent containing two carboxylic acids joined together at a secondary amine. The carboxylic acids are reactive towards primary amines in the presence of activators such as HATU or EDC.

Fmoc-Amido-Tri-(carboxyethoxymethyl)-methane is a multifunctional reagent containing three carboxylic acids and a primary amine that is Fmoc-protected. The carboxylic acids can react with primary amine groups to form stable amide bonds. The Fmoc protecting group can be removed using piperidine, freeing up the amine for a variety of uses such as coupling with carboxylic acids to form amide bonds.

Biotin-PEG2-alkyne is a short PEG linker featuring a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azides.

endo-BCN-PEG8-PFP ester is a PEG linker featuring a BCN group and a PFP ester. BCN is a click chemistry handle used to react with azides on target molecules while the PFP is a good leaving group which is readily displaced by amines to form amides.