PEG

N-(Amino-PEG4)-N-bis(PEG4-Azide) is a three-armed PEG linker containing two azide groups and a primary amine. The two azides are free for click chemistry with terminal alkynes or strained cyclooctynes such as DBCO or BCN while the primary amine is reactive towards carboxylic acids and other carbonyl-containing functional groups. The junction combining this molecule’s branches is a tertiary amine.

2-((2-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)ethoxy)methyl)propane-1,3-diol is a small trifunctional compound which contains an azide as well as two primary alcohols. Azides are commonly applied for click chemistry with terminal alkynes, BCN, or DBCO while alcohol groups are versatile building blocks for building larger compounds. The use of a PEG linker increases this compound’s aqueous solubility, altering its DMPK properties in drug development.

N-(3-Methoxy-3-oxopropyl)-N-propyl-ß-alanine methyl ester is a branched structure eaturing two identical methyl esters and a propyl group linked by a central tertiary amine.

DNP-PEG12-amine is a bifunctional PEG linker featuring a dinitrophenyl and a primary amine. Dinitrophenyl is an excellent FRET quencher pair with tryptophan or tyrosine, while the amine can be used in a variety of ways such as reductive aminations or couplings.

N-(Amine-PEG8)-N-bis(PEG8-Propargyl) is a trifunctional linker containing an amino group and two terminal alkynes. These alkynes are frequently used in copper (I) click reactions with azides to form stable triazole linkages, while the primary amine is reactive towards carboxylic acids and other carbonyl compounds such as NHS esters, aldehydes or ketones.

DOPE-PEG-Fluor 555, MW 2,000 is a PEG-lipid-dye conjugate featuring a DOPE phospholipid and a Fluor 555 dye. DOPE is featured prominently in lipid nanoparticles for its ability to readily form micelles while Fluor 555 is a fluorescent dye which is used in histology, FRET and other fluorescence-based assays in vivo.

1-(PEG-Amine)-2,3-(PEG4-Azide) is a trifunctional linker containing two azides and a primary amine. This amine can be used to couple with carboxylic acids in the presence of an activator such as HATU or EDC, while the azides are open to do click chemistry with terminal alkynes or strained cyclooctynes. PEG linkers are highly water soluble and altering chain length will impact DMPK in drug discovery applications.

Azido-PEG8-(CH2)3OH is a bifunctional PEG linker featuring an azide and alcohol. Azides are click chemistry handles which readily react with terminal alkynes or strained cyclooctynes while the hydroxy group is a versatile functionality which may be employed in a wide variety of reactions.

6-Formyl-2-pyridine-PEG4-DBCO is a bifunctional PEG linker featuring an aldehyde and a DBCO function. DBCO is a strained cyclooctyne which reacts with azide groups on target molecules while the aldehyde group is a versatile electrophile that is reactive towards amines, hydroxylamines, and hydrazines.