PEG

1-Pyrrolidinebutanoic acid, HCl salt

Ald-Ph-PEG8-NHS ester is a PEG linker containing a benzaldehyde and an NHS ester. The aldehyde group is a versatile electrophile that is reactive towards amines, hydroxylamines, and hydrazines while the NHS ester may easily react with amines or alcohols to form amides or esters.

Biotin-PEG11-TCO is a PEG linker containing a biotin group and a trans-cyclooctene (TCO) functional group. Biotin is useful for affinity-based applications such as pull-down assays while TCO is a click chemistry handle that quickly reacts with tetrazine groups on target molecules.

Dehydroergosterol (DHE), a phytosterol derived from ergostane, contains multiple double bonds at positions 5,6-, 7,8-, 9,11-, and 22,23-, along with a 3beta-hydroxy group. It is a highly rigid C-24 methyl derivative with one double bond in the tail region and two double bonds in the body region. Being a C-24 alkyl derivative, its tail and body variances influence the size, mRNA encapsulation efficiency (EE), and transfection efficiency of lipid nanoparticles (LNPs).

sulfo-Cyanine5 dimethyl is a non-functionalized, water-soluble cyanine dye with bright far-red fluorescence. The dye can be used as a non-reactive fluorophore for control experiments, calibration, and other technical applications.

NH-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are joined together at a secondary amine. Terminal alkynes are reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound. The secondary amine joining the two arms can be used as a nucleophile such as in alkylation via reductive amination or in forming amides with carboxylic acids or activated NHS esters. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.

Azide-PEG4-sulfonic acid is a bifunctional PEG linker containing terminal azide and sulfonic acid groups. Azide (N3) group can react with BCN, DBCO, and alkyne via Click Chemistry. The sulfonic acid group can participate in esterification, halogenation, and replacement reactions. The PEG spacer will improve solubility of the compound in aqueous media.

OF-C4-Deg-Lin is an ionizable lipid featuring a central piperazine-2,5-dione and two identical branched amines, each with two linoleic esters attached. This compound may be used in the preparation of lipid nanoparticles which can encapsulate nucleic acids and efficiently deliver them to target cells.

N-(acid-PEG4)-N-bis(PEG4-azide) is a branched linker containing two azides and a terminal caboxylic group. The azide group is reactive towards terminal alkynes and strained cyclooctynes using click chemistry. The terminal carboxylic acid group can conjugate with amine molecules in the presence of activators such HATU. PEG linkers provide high aqueous solubility, and altering PEG length can alter DMPK.