Lentivirus

PRAME-Specific TCR Lentivirus are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with a TCR (T cell receptor) that specifically recognizes the human antigen PRAME (Preferentially Expressed Antigen in Melanoma) peptide 425-433 (SLLQHLIGL), and in which the TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker.

PRAME-Specific TCR Lentivirus are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with a TCR (T cell receptor) that specifically recognizes the human antigen PRAME (Preferentially Expressed Antigen in Melanoma) peptide 425-433 (SLLQHLIGL), and in which the TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker.

GCaMP is a genetically encoded calcium indicator (GECI) and created from a fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light chain kinase. When GECI binds Ca2+, the conformational change of GCaMP induces proximity of the GFP parts, eliciting strong GFP fluorescence signal, which allows measuring action potentials and other receptor activation events that trigger Ca2+ influx. The advantage of GECI's are that they can be genetically specified for studies in living organisms. GCaMP6 is a novel ultra-sensitive format of GCaMP that outperformed other sensors in terms of accuracy and sensitivity in measuring cytosol Ca2+ level. Mutations in calmodulin part of GCaMP6 created several variants which showed different calcium fluorescence decay rate (fast - GCamP6f, slow - GCaMP6f and medium - GCaMP6m). LV-CaMKII-GCaMP6f is pre-made lentivirus which expresses GCaMP6f under CaMKII promoter for neuronal exclusive expression. Ready to us