Cells Reagents

Targetmol RIPA Lysis Buffer is a traditional rapid lysis buffer for cellular tissues. RIPA stands for Radio Immunoprecipitation Assay. There are many different formulations of RIPA lysis buffer, which can be broadly categorized into strong, medium, and weak depending on the strength of the lysate. The protein samples obtained from the buffer can be used for general PAGE, Western Blot (WB), Immunoprecipitation (IP), Co-immunoprecipitation (Co-IP) and ELISA. This product can be used for cell or tissue samples of animals and plants, as well as fungal or bacterial samples. The main components of a weak RIPA lysis buffer include 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, and sodium orthovanadate, sodium fluoride, EDTA, leupeptin, and many other inhibitors. However, our RIPA lysis buffer does not contain all of the components of a protease/phosphatase inhibitor Cocktail. To effectively prevent protein degradation, adding PMSF or Protease/Phosphatase Inhibitor Cock

TargetMol's Mouse CD3+ T Cell Isolation Kit (negative selection) provides superparamagnetic beads for the isolation of CD3+ T cells from single-cell suspensions of mouse splenocytes or other tissues using a negative sorting method. The principle is to use different biotin-labelled monoclonal antibodies to label non-target cells (non-CD3+ T cells), which are then cleared by streptavidin-labelled magnetic beads for the purpose of mouse CD3+ T cell sorting.

TargetMol's Mouse CD4+ T Cell Isolation Kit (negative selection) provides superparamagnetic beads for the isolation of CD4+ T cells from single-cell suspensions of mouse splenocytes or other tissues using a negative sorting method. The principle is to use different biotin-labelled monoclonal antibodies to label non-target cells (non-CD4+ T cells), which are then cleared by streptavidin-labelled magnetic beads for the purpose of mouse CD4+ T cell sorting.

TargetMol's Mouse CD8+ T Cell Isolation Kit (negative selection) provides superparamagnetic beads for the isolation of CD8+ T cells from single-cell suspensions of mouse splenocytes or other tissues using a negative sorting method. The principle is to use different biotin-labelled monoclonal antibodies to label non-target cells (non-CD8+ T cells), which are then cleared by streptavidin-labelled magnetic beads for the purpose of mouse CD8+ T cell sorting.

TargetMol's Mouse Neutrophil Cell Isolation Kit (negative selection) provides superparamagnetic beads for the isolation of neutrophils from single-cell suspensions of mouse bone marrow or other tissue samples by negative sorting. The principle is to use different biotin-labelled monoclonal antibodies to label non-target cells, which are then cleared by streptavidin-labelled magnetic beads for the purpose of mouse Neutrophil cell sorting.

TargetMol's Human CD3+ T Cell Isolation Kit (negative selection) provides superparamagnetic beads for the isolation of CD3+ T cells from human peripheral blood mononuclear cells (PBMC) using a negative sorting method. The principle is to use biotin-labelled monoclonal antibody to label non-target cells (non-CD3+ T cells), and then remove the non-target cells by streptavidin-labelled magnetic beads for the purpose of human peripheral blood CD3+ T cell sorting.

TargetMol's Human CD34+ T Cell Isolation Kit (negative selection) provides superparamagnetic beads for the isolation of CD34+ T cells from human peripheral blood mononuclear cells (PBMC) using a negative sorting method. The principle is to use biotin-labelled monoclonal antibody to label non-target cells (non-CD34+ T cells), and then remove the non-target cells by streptavidin-labelled magnetic beads for the purpose of human peripheral blood CD34+ T cell sorting.

TargetMol's Mouse CD4+ Cell Isolation Kit (positive selection) provides superparamagnetic beads covalently coated with a monoclonal antibody specific for CD4 membrane antigens expressed predominantly on murine helper/inducer T cells, based on the principle of labelling CD4+ cells with the CD4 Capture Antibody, then capturing the target cells with Releaseable Beads. The principle is to use CD4 Capture Antibody to label CD4+ cells, then capture the target cells by Releasable Beads, and then dissociate the beads from the cell surface by Beads Release Buffer, thus obtaining mouse CD4+ cells without bead labelling. This rapid and gentle method eliminates the need for a column and helps ensure the purity, recovery and viability of the isolated CD4+ cells. The sorted CD4+ cells can be used in downstream molecular and cell biology experiments.

TargetMol's Mouse CD8+ Cell Isolation Kit (positive selection) provides superparamagnetic beads covalently coated with a monoclonal antibody specific for CD8 membrane antigens expressed predominantly on murine helper/inducer T cells, based on the principle of labelling CD8+ cells with the CD8 Capture Antibody, then capturing the target cells with Releaseable Beads. The principle is to use CD8 Capture Antibody to label CD8+ cells, then capture the target cells by Releasable Beads, and then dissociate the beads from the cell surface by Beads Release Buffer, thus obtaining mouse CD8+ cells without bead labelling. This rapid and gentle method eliminates the need for a column and helps ensure the purity, recovery and viability of the isolated CD8+ cells. The sorted CD8+ cells can be used in downstream molecular and cell biology experiments.