Results for Other Proteins ( 58831 )
- From: €209.00
Goat IgG whole molecule Agarose Conjugated product is made from IgG, secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
- From: €161.00
For use in “pre-clearing” cell lysates, as well as a negative control for the pulldown of polyubiquitylated proteins in conjunction with agarose-conjugated TUBEs (UM401 and UM402). Control Agarose is generated by blocking an activated matrix (identical to that used for TUBEs conjugation) with ethanolamine. Control Agarose (200µl resin) is supplied as a 50% slurry in PBS, pH 7.2, 20% ethanol.
- From: €1,561.00
Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” protein in its polyubiquitin state. TUBE 2 is based on UBA1 from human RAD23A. Agarose coupled TUBEs allows for efficient recovery of polyubiquitinated proteins in a single step. Polyubiquitinated protein can easily be separated from TUBEs for proteomic studies or for identification by western blotting.