Other Proteins

Recombinant Mouse Macrophage Inflammatory Protein-1 alpha produced in E. coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7820 Daltons.

Interleukin-1beta (IL-1β) is a proinflammatory cytokine produced in response to inflammatory agents by a variety of cells, including monocytes, macrophages, and dendritic cells (DCs). IL-1β and IL-1α are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1α is cell associated and IL-1β is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Recombinant mouse IL-1β is a non-glycosylated single chain 17,394 Da protein containing 152 amino acid residues.

Interleukin-1beta (IL-1β) is a proinflammatory cytokine produced in response to inflammatory agents by a variety of cells, including monocytes, macrophages, and dendritic cells (DCs). IL-1β and IL-1α are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1α is cell associated and IL-1β is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Recombinant mouse IL-1β is a non-glycosylated single chain 17,394 Da protein containing 152 amino acid residues.

Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. In addition to its critical role in the physiologic regulation of body weight leptin has a variety of other physiologic and pathologic functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. Recombinant Mouse Leptin produced in E. coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.1 kDa.

Tumor Necrosis Factor-alpha (TNF-a) is pleiotrophic cytokine secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following stimulation by bacterial LPS . TNF-a activity is mediated via interactions with TNFR1 &TNFR2 receptors. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2 & GM-CSF. Recombinant mouse TNF-a produced in E.Coli is a non-glycosylated polypeptide chain containing 156 amino acids and having a molecular mass of 17,300 daltons.

Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda.

Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda. IgG1 is the most abundant of the four IgG subclasses. This isotype control possesses lambda light chains.

Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda.

Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma- IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda.