Other Proteins

Sonic hedgehog (SHH) is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology and all signal via the same receptors. Recombinant mouse SHH is a non-glycosylated protein, containing 176 amino acids, with a molecular weight of 19.8 kDa. The Cys at position 25 has been substituted with Ile.

Sonic hedgehog (SHH) is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology and all signal via the same receptors. Recombinant mouse SHH is a non-glycosylated protein, containing 176 amino acids, with a molecular weight of 19.8 kDa. The Cys at position 25 has been substituted with Ile.

Thrombopoietin (TPO) is a growth factor that is produced by the liver and kidney. TPO acts through the TPO receptor to promote megakaryocyte maturation and differentiation, which leads to the production of platelets. Recombinant mouse TPO is a non-glycosylated protein, containing 174 amino acids (which comprise the receptor binding domain), with a molecular weight of 18.7 kDa.

Thrombopoietin (TPO) is a growth factor that is produced by the liver and kidney. TPO acts through the TPO receptor to promote megakaryocyte maturation and differentiation, which leads to the production of platelets. Recombinant mouse TPO is a non-glycosylated protein, containing 174 amino acids (which comprise the receptor binding domain), with a molecular weight of 18.7 kDa.

Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilation via nitric oxide-dependent pathways. Mouse VEGF-A has several variants, one being VEGF-120. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95%. Recombinant mouse VEGF-120 is an alternative spliced version of mouse VEGF-165. It is a non-glycosylated homodimer, containing two chains, with a molecular weight of 28.2 kDa.

Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilation via nitric oxide-dependent pathways. Mouse VEGF-A has several variants, one being VEGF-120. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95%. Recombinant mouse VEGF-120 is an alternative spliced version of mouse VEGF-165. It is a non-glycosylated homodimer, containing two chains, with a molecular weight of 28.2 kDa.

Isotype control Mouse IgG1 is important for Flow Cytometry. Mouse IgG1 control has no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.

Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.

Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.