10 most popular antibodies in neuroscience research

Published on December 2, 2015

Analysis of neuronal cell fates and biological activities sometimes relies on the effective staining of a protein of interest (a.k.a. marker) with a specific primary antibody validated for immuno-assays. These markers are used for labeling specific neuron lineages and understand their roles in physiological and various neurological disorders (e.g. Alzheimer’s disease, Parkinson’s disease and Amyotrophic lateral sclerosis). The choice of guaranteed antibodies is crucial. In this post, a selection of the most popular primary antibodies used in neuroscience research and used by researchers all over the world is made. This selection of high quality research antibodies is illustrated with experimental results obtained from skilled scientists.

Anti SR-1A (F312): a Rabbit Polyclonal antibody to 5-hydroxytryptamine receptor 1A compatible with WB, IHC and IF (Bioworld Technology – cat. nr 281S2180)
Anti NCALD: a Rabbit Polyclonal antibody to Neurocalcin-delta for WB applications (Bioworld Technology – cat. nr281BS61223)
Anti CALB2: a Rabbit Polyclonal antibody to Calretinin / Calbindin 2 for WB, IHC and IF applications (Abnova – cat. nr 157PAB20372)
Anti GFAP: a Chicken Polyclonal antibody to Glial Fibrillary Acidic Protein for WB & IF applications (Rockland Immunochemicals – cat. nr 039200-901-D60)
Anti Prph: a Chicken Polyclonal antibody to Peripherin for WB and IC. A dilution of 1:5,000 – 1:10,000 is recommended for WB (BioSensis – cat. nr 211C-1399-50)
Anti PAX6 (1C8): a Rabbit Polyclonal antibody to PAX6 (paired box 6) for WB, IHC, ELISA and IF (Abnova – cat. nr 157MAB10293)
Anti ENO1 (8G8): a Monoclonal antibody to ENO1 (Enolase 1 or alpha-enolase (Abnova – cat. nr 157H00002023-M01) compatible with WB, IHC and IF
Anti Aβ (MOAB2): a Mouse Monoclonal antibody to Amyloid beta peptide (A beta 40/42) to be used in WB/IP, IHC, IF and ELISA (BioSensis – cat. nr 211M-1586-100)
Anti Tau pS416: a Rabbit Polyclonal Antibody to Tau microtubule-associated protein for WB analysis (Rockland Immunochemicals – cat. nr 039612-401-E42)
Anti NTNG2 (4F11): a Mouse Mononclonal Antibody to Netrin-G2 involved in axon guidance (Abnova – cat. nr 157H00084628-M01) for WB, IHC and ELISA

ihc-detection-of-a%ce%b2-and-app-in-coronal-sections-of-the-frontal-cortex-of-5xfad-mice-biosensis-cat-nr-211m-1586-100-figure-courtesy-of-youmans-et-al-2012 — IHC detection of Aβ and APP in coronal sections of the frontal cortex of 5xFAD mice (BioSensis, cat. nr 211M-1586-100). Figure courtesy of Youmans et al (2012)

IHC of mouse kidney using CALB2 antibody at dilution of 1100 (cat. nr 281BS71327). Source Bioworld Technology tebu-bio

IHC of mouse lung using CALB2 antibody at dilution of 1100 (cat. nr 281BS71327). Source Bioworld Technology tebu-bio

IHC staining of human cerebellum with CALB2 polyclonal antibody (cat. nr 157PAB20372). Source Abnova tebu-bio

IHC staining of MOAB-2 on 5XFAD mouse hippocampal sections with anti Aβ (BioSensis cat. nr 211M-1586-100 Red). Photo courtesy of Dr. Mary Jo LaDu and Dr. Leon Tai University of Illinois

Rat mixed neuronglial cultures stained with Chicken polyclonal antibody to Peripherin (BioSensis – cat. nr 211C-1399-50 (green) – DNA in blue.

WB analysis of extracts of various cells using CALB2 antibody (cat. nr 281BS71327). Source Bioworld Technology tebu-bio

WB of Rabbit anti-Tau pS416 (cat. nr 039612-401-E42). Source Rockland tebu-bio

Western blot analysis using PAX6 monoclonal antobody, clone 1C8 (cat. nr 157MAB10293) against recombinant human PAX6 protein. Source Abnova tebu-bio

fcm-analysis-of-3t3-l1-cells-using-pax6-monoclonal-antobody-clone-1c8-cat-nr-157mab10293-green-and-negative-control-purple-source-abnova-tebu-bio — FCM analysis of 3t3 l1 cells using PAX6 monoclonal antibody (clone 1c8) (cat. nr 157mab10293 – green and negative control purple). Source: abnova tebu-bio

IF analysis of monoclonal antibody to ENO1 (cat. nr 157H00002023-M01) on HeLa cells. Source Abnova tebu-bio

IF detection of Aβ in coronal sections of 3xTg mice stained with anti-Aβ (BioSensis, cat. nr 211M-1586-100). Figure courtesy of Youmans et al (2012)

IF Microscopy with Anti-GFAP (cat. nr 039200-901-D60 in red) of mixed cultures of neurons and glia (DNA in blue). Source Rockland tebu-bio

IF staining of human cell line U-2 OS with CALB2 polyclonal antibody (cat. nr 157PAB20372). Source Abnova tebu-bio

IHC analysis of monoclonal antibody to ENO1 (cat. nr 157H00002023-M01) on formalin-fixed paraffin-embedded human lymphoma tissue. Source Abnova tebu-bio

IHC analysis of monoclonal antibody to NTNG2 monoclonal antibody (M01), clone 4F11 (cat. nr 157H00084628-M01) on formalin-fixed paraffin-embedded human lung cancer. Source Abnova tebu-bio

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