With more than 170,000 publications in 2016, inflammation remains a “hot” topic in today’s Life Science research (source: Google Scholar). Interestingly, the ELISA test is still one of the most popular tools for the accurate quantification of human cytokines involved in this biological process. Here, we review the 5 protein targets most studied by our clients, together with their favourite ELISA kits by RayBiotech in the field of inflammation.
Target # 1 – Human Interleukin-6 (IL-6)
IL-6 is active in inflammation (either acute or chronic), and in B cell maturation. This cytokine is implicated in a wide variety of inflammation-associated disease states (incl. diabetes mellitus, rheumatoid arthritis…). To quantify human IL-6 in cell culture supernatants, plasma, and serum samples, researchers use RayBiotech‘s Human IL-6 Sandwich-based ELISA kit (cat. nr ELH-IL6-1).
This RayBio® Human IL-6 ELISA Kit has a sensitivity of 3 pg/ml (detection range: 3 pg/ml – 1,000 pg/ml) with no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
For Lysates, it is recommended to use the RayBio® Human IL-6 ELISA Kit for cell and tissue lysate samples (cat. nr ELH-IL6-CL-1).
Target # 2 – Human TNF-alpha
TNF-alpha is a proinflammatory cytokine that regulates multiple biological processes (incl. cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation). TNF is implicated in a variety of diseases such as autoimmune diseases, insulin resistance, and cancer.
The RayBio® Human TNF alpha ELISA Kit (cat. nr ELH-TNFa-1) is for cell culture supernatants, plasma, and serum samples with a sensitivity of 30 pg/ml with a detection range between 30 pg/ml and 6000 pg/ml). For Lysates, you will likely prefer the format dedicated for such biological samples: cat. nr ELH-TNFa-CL-1.
The Human TNF-a ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1 alpha, MIP-1 beta, NAP-2, PDGF, PF-4.
Target # 3 – IL1-beta
IL1-BETA is an important mediator of inflammatory response and is involved in a variety of cellular activities (incl. cell proliferation, differentiation, and apoptosis). The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the CNS contributes to inflammatory pain hypersensitivity. This cytokine has also been described to be active in cancer / immune cell crosstalk a.k.a. immuno-editing.
The RayBio® Human IL-1 beta (IL-1 F2) ELISA Kit is for cell culture supernatants, plasma, and serum samples with a sensitivity of 0.3 pg/ml (detection range: 0.3 pg/ml – 100 pg/ml; cat. nr ELH-IL1b-1).
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Target # 4 – IL-1 R4 / ST2 (IL-33 receptor)
IL-33 receptor or ST2 (IL-1 R4) when bound to IL-33 elicits either pro- or anti-inflammatory effects depending on the disease and the model. IL-33 is also considered to be a promising cardiovascular biomarker.
The RayBio® Human IL-1 R4 / ST2 (IL-33 receptor) ELISA Kit is for cell culture supernatants, plasma, and serum samples (2 pg/ml as sensibility threshold and with 2 pg/ml – 1,200 pg/ml as a detection range – cat. nr ELH-IL1R4-1).
This ELISA kit shows no cross-reactivity with the following cytokines tested: Human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Target # 5 – Interleukin-10 (IL-10) (Cytokine Synthesis Inhibitory Factor or CSIF)
IL-10 is a potent anti-inflammatory cytokine with an important role in limiting host immune response to pathogens, and to prevent damage to the host while maintaining normal tissue homeostasis.
The RayBio® Human IL-10 ELISA Kit (for cell culture supernatants, plasma, and serum samples – cat. nr ELH-IL10-1; for lysates – cat. nr ELH-IL10-CL-1) are sensitivite (1 pg/ml) with a detection range from 1 pg/ml to 150 pg/ml.
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Why use such RayBio® ELISA kits for inflammation studies?
The RayBio® Sandwich-based ELISA kits are affordable in vitro enzyme-linked immunosorbent assays for the quantitative measurement of soluble proteins (biomarker) in a variety of species.
To date, 2,500+ ELISA kits to quantify cytokines, growth factors, proteases, soluble receptors, apoptosis effectors, and many other soluble proteins, are available and are shipped under strip plate formats for use in multiple experiments.
They are ideal companions for:
- Detection of quantitative protein levels in biological fluids
- Validation of biomarker discovery studies
- Confirmation of results obtained with RayBiotech’s Antibody array focusing on inflammation such as:
- Inflammation C-Series: Membrane-Based Antibody Arrays to detect 20 Human Inflammatory Factors from all liquid sample types (Serum, Plasma, Cell Culture Supernatants, Cell Lysates, Tissue Lysates).
- Inflammation G-Series: A 75mm x 25mm glass slide chip that delivers easier handling along with the use of much lower sample volumes (10-100 µl per array). The glass slide also utilizes a fluorescent signal readout, allowing a wider dynamic detection range than can be achieved by chemiluminescence.
- Quantibody®: Multiplex ELISA Array Quantitative, Sandwich-based, Glass Slide Antibody Arrays, an array-based multiplex ELISA system for simultaneous quantitative measurement of multiple cytokines involved in inflammation.
Not enough time to use RayBiotech’s kits yourself?
Think about outsourcing your biomarker discover & validation programs!
All the items described above for your inflammation studies are available as ready-to-use kits or lab services. This means that skilled technicians and project managers can perform the experiments for you so that you can focus on other valued research projects. To ensure top quality in the data obtained during outsourcing strategies, RayBiotech’s service providers successfully complete training and certification programs to receive the “RayBiotech Certified Array Service Providers” award.
In Europe, tebu-bio’s laboratories (France) were among the first laboratories in the world to be certified by RayBiotech in 2012 for Protein profiling and Protein Multiplex Quantification.
