2 new validated anti-PGP 9.5 antibodies for neuroscience research

Published on October 12, 2016

Protein Gene Product 9.5 (PGP 9.5) is an abundant cytoplasmic neuron and neuroendocrine-cell specific protein. Over the years, this deubiquitinating enzyme (a.k.a. Ubiquitin Carboxy-terminal Hydrolase L1 (UCH-L1)) has become an accepted biomarker for neurons and cells of the diffuse neuroendocrine system (DNES) but also when studying neurodegenerative disorders (e.g. Alzheimer’s and Parkinson’s diseases) and cancers.

Research antibodies specific for PGP9.5 / UCH-L1 stain neuronal cell bodies and axons in the central and peripheral nervous systems, small nerve fibres in nerve tissue, neuroendocrine cells in the pituitary, thyroid pancreas, and also tumours of the DNES.

In this context, Cedarlane just release 2 new anti-PGP9.5 / UCHL1 rabbit polyclonal antibodies fully validated & guaranteed for IHC analysis of Paraffin Embedded Sections, IF and WB applications on Human, Rat and Mouse tissues.

validtion-by-if-of-the-new-anti-pgp9-5-polyclonal-cat-nr-007cl7756ap-cedarlane-tebu-bio — PGP9.5 staining of a rat paw tissue section by IF with the new anti-PGP 9.5 cat. nr 007CL7756AP. Intraepithelial nerve staining of PGP 9.5 is observed (green; DAPI in blue). Courtesy of Michael Anderson (Oklahoma State University).

validtion-by-ihc-of-the-new-anti-pgp9-5-polyclonal-cat-nr-007cl7755ap-cedarlane-tebu-bio — PGP 9.5 staining of a human cerebral cortex formalin-fixed, paraffin-embedded tissue section (10x (left) and 20x (right) magnification) with the new anti-PGP 9.5 cat. nr 007CL95101. Staining on normal brain cortex is negative. High grade tumor (glioma) shows sharp nuclear and cytoplasmic staining in more than 70% of the lesion. Source: Cedarlane |tebu-bio.

validation-by-wb-of-the-new-anti-pgp9-5-polyclonal-cat-nr-007cl7756ap-cedarlane-tebu-bio — PGP9.5 staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35µg protein in RIPA buffer) with the new anti-PGP 9.5 cat. nr 007CL7756AP. Source: Cedarlane |tebu-bio.

Learn more about Cedarlane’s 2 new Anti-Human PGP 9.5 (UCHL1) primary antibodies

Anti-Human PGP 9.5 (UCHL1) (199), (affinity purified) (Rabbit IgG) – cat. nr 007CL7755AP
Anti-Human PGP 9.5 (UCHL1) (184), (affinity purified) (Rabbit IgG)- cat. nr 007CL7756AP

10 related popular reagents for neuroscience research

Mouse monoclonal antibody to UCHL1 [clone BH7] (cat. nr 211M-1407-100 (Biosensis) for WB, IF & IHC)
Rabbit polyclonal antibody to Neurofilament Heavy (NH-200) (cat. nr 211R-1388-50 (Biosensis) for WB, IF)
Rabbit Polyclonal Antibody to Microtubule-associated protein tau (cat. nr 281BS3639 – Bioworld Technology)
Amylo-Glo® RTD™ (cat. nr 211TR-300-AG), a bright blue UV excitable ready-to-dilute reagent to stain Aß plaques in tissue sections
UCHL1 (PGP9.5) Active Human Recombinant Protein (cat. nr 14980351 – BPS Bioscience)
Ubiquitin-Rhodamine (cat. nr 182SI230 – LifeSensors)
GBR-12909 2HCl (CAS: 67469-78-7) | Dopamine reuptake inhibitor (cat. nr 21910-1458 – Focus Biomolecules)
GNE-7915 (CAS: 1351761-44-8) | Selective, and brain-penetrable leucine-rich repeat kinase 2 (LRRK2) inhibitor (cat. nr 282T1945 – Focus Biomolecules)
CNS Library containing a collection of 600 compounds related to CNS and targeting 5-HT receptor, AChR, Histamine receptor, dopamine receptor, Opiod receptor… (cat. nr 282L2600 – TargetMol)
RayBiotech’s Neuro Discovery C-Series Arrays for simultaneaous detection and semi-quantification of 20 (cat. nr 126AAH-NEU-1) or 30 (cat. nr 126AAH-NEU-2) neurologically relevant cytokine