When preparing a protocol for Western blotting phosphorylated proteins, a few essential points should be taken into consideration to obtain accurate immunoblotting results. Your regular WB protocol, such as the WB protocol edited by tebu-bio’s antibody users, can be fine-tuned according to this “key” post-translational protein modification.
We’ve put together 5 tech tips, which are sure to support you in designing your phosphorylated protein Western blot:
- Check phospho-specificity of primary antibodies used in Western blot
- Pamper your phospho-proteins in your samples
- Use blotting buffers compatible with phosphorylation studies by Western Blot
- Enrich your samples to detect low abundance proteins
- Stripping and re-probing your Western Blot membrane for using another antibody
Download the Guide
“5 Tips for Phosphorylated Western Blot”
