CRISPR-Cas9 is a popular method that brings researchers endless experimental strategies to create their own research-based cellular models. In this post we’ll review a new transfection reagent especially engineered to maximize Cas9 vectors deliveries inside cells with low cellular toxicity.
Whatever the experimental objectives (gene knock-out or knock-in, single point mutation(s), promoter swapping, protein truncation…) and applications (Drug discovery, Cell signaling, Phenotypic assays…), CRISPR gene editing methods require extremely robust reagents (cell lines and sgRNA, Cas9 mRNA, anti-Cas9 or anti-GFP antibodies, …).
MTI-GlobalStem has released a new high quality transfection reagent to deliver Cas9 expression vectors or Cas9/sgRNA expression vectors: the DNA-In® CRISPR transfection reagent.
DNA-In® CRISPR has been developed for the delivery of large CRISPR/Cas9 plasmid expression vectors into a broad range of cell types. Successfully used on primary cells and cell lines (Human primary fibroblasts, keratinocytes, endothelial cells (HuVEC), skeletal muscle cells, HeLa cells and C2C12 mouse myoblasts… to name but a few!), the animal-free DNA-In® CRISPR achieves the highest number of cells transfected in a population without toxicity.
This reagent is ideal for intracellular delivery of DNA into cells in the presence of serum at a cell density of 50% to 70%.
Download the optimized DNA-In® CRISPR Transfection Reagent protocol here.
Want to know more about DNA-In® CRISPR Transfection Reagent? Looking for tailored made solutions in relation to your CRISPR/CAS9-based experiments? Leave us a message below.
tebu-bio’s exoerts advise you in the design of your genome editing experiments and provide you with user-friendly solutions suited to your cellular models.
