Live cell imaging: the art of looking at biological processes in real time

Visualizing fixed cells and tissues only gives snapshots of cellular processes. To have a better insight into dynamic events ocurring in the cells or to vizualize interactions between various cellular components in real time (e.g., proteins, organelles, second messengers…), powerful microscopic approaches have been developed over the past decade. This post will review the recent live cell imaging probes developed by Goryo Chemicalsand available in Europe through tebu-bio.

In 2014, the Nobel price in chemistry was attributed to Eric Betzig, Stefan W. Hell, and William E. Moerner “for the development of super-resolved fluorescence microscopy” opening a new age for Live Cell Imaging. Besides elucidating intimate interactions of various cellular components (e.g. Actin, Tubulin and DNA), molecular probes used in live cell imaging allow to monitor accurately biological changes of various parameters in living cells (such as ion concentration variations or any other changes in the cytosol).  tebu-bio’s specialists are constantly searching for innovative and biologically relevant probes for live cell imaging applications.

tebu-bio is now collaborating with Goryo Chemical to distribute in Europe their unique fluorescent dye for cellular analysis, assay, and live cell imaging.

Below a selection of the new fluorescent probes:

AcidiFluor™ Series

• pH probe for Acidic Organelle Imaging
• Fluorescent reagent for analyzing phagocytosis
• endocytosis detection beads
• pH probe for labeling proteins and nucleic acids

CalFluor™ Series

• red fluorescent Ca2+ probes
• near-infrared Ca2+ probes
• water soluble Ca2+ probes

GlycoFluor™ Series

• Imaging of β galactosidase
• Imaging of β-glucosidase
• Imaging of β-glucuronidase

MetalloFluor™ Series

• Detection of Fe2+
• Detection of  Zn2+

NOFluor™ Series

• Probes to measure Nitric oxide (NO) generated by tissues or cells

ROSFluor™ Series

• Probes to measure reactive oxgen species (ROS)
• Probes to measure peroxynitrite (ONOO－)

ProteoFluor™ Series

• Cancer cells detection probe

StemFluor™ Series

• Chemical Probe for human iPS/ES cells  –  Kyoto probe 1 – see previous blog: Live Cell Imaging: Differentiate ES/iPS from differentiated cells

HypoFluor™ Series

• Hypoxia detection probe

SuperFluor™ Series

• Fluorescence probe for super-resolution live imaging against Halo-tag

If you are interested in testing one of the live cell imaging stains presented, please leave your request in the form below.

You might also like to get a complete overview about the unique tebu-bio’s Live cell imaging offer via an interactive Live cell imaging tool selection guide! On this guide, all you need is just to click on the biological parameter you want to visualize – and you’ll find the corresponding molecular probe available.

Interested in learning more about tools like this?
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