Quick & easy ROS detection tools!

Reactive oxygen species (ROS) are primarily produced in mitochondria during ATP synthesis. ROS plays an essential role in signaling pathways and the immune system, whereas increasing ROS is associated with diseases and cellular senescence.

ROS analysis is an essential approach to understanding the cause of various cellular processes such as ferroptosis.

Products	ROS Assay Kit – Highly Sensitive DCFH-DA-	ROS Assay Kit – Photo-oxidation Resistant DCFH-DA-
Item#	R252-10	R253-10
Feature	Detect ROS in cells. High sensitivity.	Detect ROS in cells. High retention.
Machine	Microscope, FCM, Plate Reader	Microscope, FCM, Plate Reader
Photo-oxidation resistant	–	+++
Cell fixation	–	+++
Sensitivity	+++	++

R252-10 function

R253-10 function

Time-lapse imaging with R253 assay kit

RAW264.7 cells are stained with ROS Assay Kit -Photo-oxidation Resistant DCFH-DA- (R253) stained. After LPS (Lipopolysaccharide)-was treated, the intracellular ROS level was simultaneously observed with a fluorescence microscope.

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Oxidative Stress Toolbox

Go further and learn about relationship between antitumor effects and active oxygen

References

S. Kato, “Effects of platinum‑coexisting dopamine with X‑ray irradiation upon human glioblastoma cell proliferation”, Hum. Cell, 2021, doi:10.1007/s13577-021-00591-3.
K. Kanno, T. Sakaue, M. Hamaguchi, K. Namiguchi, D. Nanba, J. Aono, M. Kurata, J. Masumoto, S. Higashiyama, H. Izutani, “Hypoxic Culture Maintains Cell Growth of the Primary Human Valve Interstitial Cells with Stemness“, Int. J. Mol. Sci.2021, doi:10.3390/ijms221910534.
C. Fang, L. Wu, M. Zhao, T. Deng, J. Gu, X. Guo, C. Li, W. Li, X. Zeng, “Periodontitis Exacerbates Benign Prostatic Hyperplasia through Regulation of Oxidative Stress and Inflammation”, Oxid. Med. Cell. Longevity, 2021, doi:10.1155/2021/2094665.
M. Tamoi and S. Shigeoka, “CP12 Is Involved in Protection against High Light Intensity by Suppressing the ROS Generation in Synechococcus elongatusPCC7942″, Plants, 2021, doi:10.3390/plants10071432.
S. Xia, Z. Ma, B. Wang, F. Gao, C. Yi, X. Zhou, S. Guo, L. Zhou, “In vitro anti-synovial sarcoma effect of diallyl trisulfide and mRNA profiling”, Gene, 2021, doi:10.1016/j.gene.2021.146172.
Z. Luo, Q. Gao, H. Zhang, Y. Zhang, S. Zhou, J. Zhang, W. Xu, J. Xu, ” Microbe-derived antioxidants attenuate cobalt chloride-induced mitochondrial function, autophagy and BNIP3-dependent mitophagy pathways in BRL3A cells”, 2022, doi:10.1016/j.ecoenv.2022.113219.
Y. Chen, Z. Wang, Y. Song, N. Chen, J. Guo, W. Liu, K. Guo, X. Ling and L. Zhang,“4‐octyl itaconate improves the viability of D66H cells by regulating the KEAP1‐NRF2‐GCLC/HO‐1 pathway”, J Cell Mol Med., 2022, doi: 10.1111/jcmm.17708.
K. Suyama, D. Sakai, S. Hayashi, N. Qu, H. Terayama, D. Kiyoshima, K. Nagahori and M. Watanabe,“Bag-1 Protects Nucleus Pulposus Cells from Oxidative Stress by Interacting with HSP70”, Biomedicines., 2023, doi: 10.3390/biomedicines11030863.
H. Liu, W. Zhang, L. Jin, S. Liu, L. Liang and Y. Wei,“Plumbagin Exhibits Genotoxicity and Induces G2/M Cell Cycle Arrest via ROS-Mediated Oxidative Stress and Activation of ATM-p53 Signaling Pathway in Hepatocellular Cells”, Int. J. Mol. Sci., 2023, doi: 10.3390/ijms24076279.
M. Sakurai, K. Ishitsuka, T. Ito, A. C. Wilkinson, T. Kimura, E. Mizutani, H. Nishikii, K. Sudo, H. J. Becker, H. Takemoto, T. Sano, K Kataoka, S. Takahashi, Y. Nakamura, D. G. Kent, A. Iwama, S. Chiba, S. Okamoto, H. Nakauchi and S. Yamazaki, “Chemically defined cytokine-free expansion of human haematopoietic stem cells”, Nature, 2023, doi:10.1038/s41586-023-05739-9.

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Quick & easy ROS detection tools!

R252-10 function

R253-10 function

Time-lapse imaging with R253 assay kit

RAW264.7 cells are stained with ROS Assay Kit -Photo-oxidation Resistant DCFH-DA- (R253) stained. After LPS (Lipopolysaccharide)-was treated, the intracellular ROS level was simultaneously observed with a fluorescence microscope.

Oxidative Stress Toolbox

Go further and learn about relationship between antitumor effects and active oxygen

References

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