Many of BPS cell lines utilize the firefly luciferase reporter system, in which the firefly luciferase gene is downstream of a response element specific to a particular cell signaling pathway. Upon transcription factor binding to the response element, luciferase RNA is transcribed and translated into a functional luciferase protein. After addition of luciferin, luciferase converts luciferin to give off light. This bioluminescent reaction is readily detected, the amount of light is proportional to the expression of luciferase.

All BPS cell lines have been validated and tested for viability and mycoplasma to ensure valid, reproducible results and to minimize the need for optimization and troubleshooting.