Pooled human hepatocytes are a preferred test system in many drug discovery and development applications requiring intact cellular systems for in vitro testing. Intact hepatocytes contain the major hepatic drug-metabolizing enzymes and co-factors required to effectively evaluate the metabolism and potential drug-drug interactions of drug candidates.
Over the last decade, improvements in cryopreservation technologies have made using cryopreserved human hepatocytes more convenient. Pooled cryopreserved hepatocytes reduce the inter-individual differences and polymorphic distribution of liver enzymes. However, it’s crucial to carefully select a pool according to its performance but also the application it is to be used for.
Reduce inter-individual differences with HepatoSure™
HepatoSure™
is Sekisui XenoTech’s 100-donor pool of cryopreserved human hepatocytes. The largest pool on the market, HepatoSure™ provides the best value when it comes to consistency of data reproducibility.
This pool is characterized for 10 CYPs activities, UGT and SULT. But as this characterization can be found with mostly any other pool… what more do you get with HepatoSure? Sekisui Xenotech have added the Aldehyde Oxidase activity, using 2 marker substrates since AO metabolism can play an extensive role in the clearance of many drugs. Intrinsic clearance values, using 5 marker substrates have been also added and finally they looked into uptake activity with 4 specific transporters.
Reducing lot-to-lot variability
Why offer a large pool if it wouldn’t be possible to offer lot-to-lot consistency? In the following graph, you can see the activities for 4 different HepatoSure™ lots. It has been shown that there is minimal variability when comparing lot-to-lot, thus explaining why HepatoSure™ was designed for assays where continued use of historical data is desired.
Increased availability
HepatoSure™ is a 100-donor pool with a large perspective of offering a high number of vials. If you intend to harmonize and to source a unique batch for multi-sites, HepatoSure™ is probably your best solution to consider.
Applications
- Met ID
- Metabolic stability
- Drug inhibition
- Uptake assays
- Metabolism by aldehyde oxidase
Finally, each order of HepatoSure™ is shipped with the HepatoSure™ Optimization Kit, a nutrient rich media which ensures consistent cell yield and the highest possible viability.
Interested? Get in touch via the form below to ask any questions, we’ll be pleased to answer!
