Poly (ADP-ribose) polymerase (PARP) is a family of proteins which catalyze the NAD-dependent addition of Poly ADP-ribose (PAR) chains to nuclear proteins. PARPs play key signaling roles in apoptosis, DNA excision and repair, telomere stability, and chromatin structure. PARPs are also involved in transcriptional regulation of several signaling pathways, including genes involved in cancer and inflammation.
PARPs are composed of four domains of interest: a DNA-binding domain which contains two zinc finger motifs, a caspase-cleaved domain, an auto-modification domain, and a catalytic domain. Upon DNA damage, the DNA-binding domain binds the damaged DNA and induce a conformational shift, subsequently the catalytic domain starts with the synthesis of a poly (ADP-ribose) chain (PAR) as a signal for the other DNA-repairing enzymes e.g. DNA ligase III. Finally the auto-modification domain is responsible for releasing the protein from the DNA after catalysis and plays an integral role in cleavage-induced inactivation.
Broadest portfolio of PARPs and PARP related products
If you’re studying enzyme kinetics or screening small molecular inhibitors for drug discovery and HTS applications, you’ll need to access PARP isozymes. BPS Biosciences provides 13 different PARP isozymes (out of 17 isozymes known). They also have a selection of 18 different kits for your activity and inhibitor screening assays, as well as a large number of PARP inhibitors and substrates. All these products have shown to be reliable and trouble-free.
Are you working on PARP related screening projects? Share any comments or ask your questions below!
