Anti-CD19-Anti-CD3-His-Tag is a purified recombinant human bispecific T cell Engager (BiTE) based on Amgen's Blinatumomab. This BiTE has been tested for specific activity in both ELISA binding assay to CD19-biotin and functional reporter assays using Jurkat/NFAT-luc or Jurkat/IL2-Luc reporter cell lines in the presence of CD19+ Raji cells.

Anti-CD19-Anti-CD3-His-Tag is a purified recombinant human bispecific T cell Engager (BiTE) based on Amgen's Blinatumomab. This BiTE has been tested for specific activity in both ELISA binding assay to CD19-biotin and functional reporter assays using Jurkat/NFAT-luc or Jurkat/IL2-Luc reporter cell lines in the presence of CD19+ Raji cells.

Please note this product may be subject to fees, we invite you to contact your local office. The NFAT Reporter - Jurkat Cell Line contains a firefly luciferase gene under the control of the NFAT response element stably integrated into Jurkat cells. This cell line has been validated for response to thapsigargin, ionomycin, and phorbol 12-myristate 13-acetate (PMA). It is useful as a control cell line for other NFAT reporter cell lines expressing various immune checkpoint receptors.

Please note this product may be subject to fees, we invite you to contact your local office. Human IL-2 reporter construct is stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by a human IL-2 promoter.

Human CD19, also known as B-lymphocyte antigen CD19, CVID3, and Cluster of Differentiation 19, GenBank Accession No. NM_001770, a.a 21-291, with C-terminal Avi-Tag™ fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. MW = 59 kDa. This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation. This protein is enzymatically biotinylated using Avi-Tag™ technology.

Human CD19, also known as B-lymphocyte antigen CD19, CVID3, and Cluster of Differentiation 19, GenBank Accession No. NM_001770, a.a 21-291, with C-terminal Avi-Tag™ fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. MW = 59 kDa. This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation. This protein is enzymatically biotinylated using Avi-Tag™ technology.

Please note this product may be subject to fees, we invite you to contact your local office. The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features: <br />• Sensitive - highly sensitive detection of firefly luciferase activity. <br />• Stable - the signal output is stable for more than two hours, providing flexibility with regard to incubation time <br />• Convenient - simple one-step, homogeneous protocol. <br />• High-throughput - one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications <br />• Compatibility

Please note this product may be subject to fees, we invite you to contact your local office. The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features: <br />• Sensitive - highly sensitive detection of firefly luciferase activity. <br />• Stable - the signal output is stable for more than two hours, providing flexibility with regard to incubation time <br />• Convenient - simple one-step, homogeneous protocol. <br />• High-throughput - one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications <br />• Compatibility

Please note this product may be subject to fees, we invite you to contact your local office. The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features: <br />• Sensitive - highly sensitive detection of firefly luciferase activity. <br />• Stable - the signal output is stable for more than two hours, providing flexibility with regard to incubation time <br />• Convenient - simple one-step, homogeneous protocol. <br />• High-throughput - one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications <br />• Compatibility - wor

Please note this product may be subject to fees, we invite you to contact your local office. The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features: <br />• Sensitive - highly sensitive detection of firefly luciferase activity. <br />• Stable - the signal output is stable for more than two hours, providing flexibility with regard to incubation time <br />• Convenient - simple one-step, homogeneous protocol. <br />• High-throughput - one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications <br />• Compatibility - wor

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal stable CHO cell line constitutively expressing full length human CD19 protein, also known as B4 or CVID3, (Genbank #NM_001770). Surface expression of CD19 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD19 expression (High, Medium, Low) to mimic different stages of cancer target cells with various CD19 expression levels.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal stable CHO cell line constitutively expressing full length human CD19 protein, also known as B4 or CVID3, (Genbank #NM_001770). Surface expression of CD19 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD19 expression (High, Medium, Low) to mimic different stages of cancer target cells with various CD19 expression levels.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal stable CHO cell line constitutively expressing full length human CD19 protein, also known as B4 or CVID3, (Genbank #NM_001770). Surface expression of CD19 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD19 expression (High, Medium, Low) to mimic different stages of cancer target cells with various CD19 expression levels.

Please note this product may be subject to fees, we invite you to contact your local office. The NFAT Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter and an antibiotic selection gene (hygromycin or puromycin) for the selection of stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity.

Please note this product may be subject to fees, we invite you to contact your local office. The NFAT Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter and an antibiotic selection gene (hygromycin or puromycin) for the selection of stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity.

Please note this product may be subject to fees, we invite you to contact your local office. The NFAT Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (hygromycin, puromycin, or G418) for the selection of stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity.