Please note this product may be subject to fees, we invite you to contact your local office. The Vγ4Vδ1 TCR NFAT-Luciferase Reporter Jurkat Cell Line was generated from the T Cell Receptor (TCR) Knockout NFAT Luciferase Reporter Jurkat Cell Line (#78556) by overexpression of human Vγ4Vδ1 TCR using lentiviral transduction (with Vγ4Vδ1 TCR Lentivirus #78986). This cell line was functionally validated with a TCR Vδ1 agonist antibody.

Please note this product may be subject to fees, we invite you to contact your local office.

Vγ9Vδ2 T Cell Expansion Kit is suitable for the ex vivo culture and expansion of human Vγ9Vδ2 T cells. γδ T cells are low abundance in PBMCs (< 2%) and therefore they need to be successfully activated and expanded to obtain adequate number of cells for γδ T-cell based immunotherapy related studies. This kit contains media and reagents necessary to drive the robust activation and expansion of the Vγ9Vδ2 T cells subpopulation from PBMCs. To improve the purity of the expanded cells, αβ T cells and B cells are depleted from the expansion culture using an antibody cocktail. This kit is provided with enough reagents and materials for the activation and expansion of Vγ9Vδ2 T cells from a starter population of 10 x 10⁷ PBMCs.

Please note this product may be subject to fees, we invite you to contact your local office. The TCR Knockout Jurkat cell line was generated by CRISPR/Cas9 genome editing to remove the TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα and β chains.

Please note this product may be subject to fees, we invite you to contact your local office. This cell line is a double knockout of TCR (T Cell Receptor) and B2M (Beta-2-Microglobulin). First, the TRAC (T-Cell Receptor Alpha Constant) and the TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from Jurkat cells to generate the TCR Knockout Jurkat cell Line (BPS Bioscience #78539). These TCR knockout cells were then used to generate a new cell line in which B2M was also genetically removed by CRISPR/Cas9 genome editing.

Please note this product may be subject to fees, we invite you to contact your local office. This cell line is a double knockout of TCR (T Cell Receptor) and B2M (Beta-2-Microglobulin). First, the TRAC (T-Cell Receptor Alpha Constant) and the TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from NFAT Luciferase Reporter Jurkat cells to generate the TCR Knockout NFAT Luciferase Reporter Jurkat cell Line (BPS Bioscience #78556). These TCR knockout cells were used to generate a new cell line in which B2M was also genetically removed by CRISPR/Cas9 genome editing.  Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity.