Please note this product may be subject to fees, we invite you to contact your local office. The AP-1 Reporter - HEK293 Cell Linee is designed for monitoring the activity of the JNK signaling pathway. The AP1 Reporter - HEK293 cell line contains a firefly luciferase gene under the control of AP1-responsive elements that are stably integrated into HEK293 cells.

Please note this product may be subject to fees, we invite you to contact your local office. The SRE Reporter - HEK293 cell line is designed for monitoring the activity of the JAK/STAT signaling pathway. The SRE Reporter - HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line.

Please note this product may be subject to fees, we invite you to contact your local office. The Gli Reporter - NIH3T3 Cell Line is designed for monitoring the activity of the hedgehog signaling pathway. The Gli Reporter - NIH3T3 Cell Line contains the firefly luciferase gene under the control of Gli responsive elements stably integrated into NIH3T3 cells. Luciferase expression correlates with activation of the hedgehog signaling pathway.

Please note this product may be subject to fees, we invite you to contact your local office. The Wnt Signaling Pathway TCF/LEF Reporter (Luc) - HEK293 Cell Line is designed for monitoring the activity of the Wnt/b-catenin signaling pathway. The Wnt Signaling Pathway TCF/LEF Reporter (Luc) - HEK293 Cell Line contains a firefly luciferase gene under the control of TCF/LEF responsive elements stably integrated into HEK293 cells. This cell line is validated for the response to the stimulation of mouse Wnt3a and to the treatment with an inhibitor of Wnt/b-catenin signaling pathway.

Please note this product may be subject to fees, we invite you to contact your local office. The RARα Reporter (Luc)-HEK293 Cell Line contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (NM_000964).

Please note this product may be subject to fees, we invite you to contact your local office. The ISRE Reporter - HEK293 Cell Line is designed for monitoring the activity of the JAK/STAT signaling pathway. The ISRE Reporter - HEK293 Cell Line contains the firefly luciferase gene under the control of ISRE stably integrated into HEK293 cells.

Please note this product may be subject to fees, we invite you to contact your local office. The ARE Reporter - Hep G2 cell line is designed to monitor Nrf2 antioxidant response pathway. The cell contains a firefly luciferase gene under the control of ARE (antioxidant response element) stably integrated into Hep G2 cells.

Please note this product may be subject to fees, we invite you to contact your local office. The cAMP/PKA Signaling Pathway CRE/CREB Reporter (Luc) - HEK293 Cell Line is designed for monitoring the activity of the cAMP/ PKA signaling pathway. The cAMP/PKA Signaling Pathway CRE/CREB Reporter (Luc) - HEK293 Cell Line contains a firefly luciferase gene under the control of multimerized cAMP response element (CRE) stably integrated into HEK293 cells. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase.

Please note this product may be subject to fees, we invite you to contact your local office. The Myc Reporter - HCT116 cell line contains the firefly luciferase gene under the control of Myc responsive elements stably integrated into HCT116 cells, a human colon cancer cell line. HCT116 contains a mutated ?beta-catenin which leads to the accumulation of β?-catenin and constitutive activation of downstream Myc that induces the expression of Myc luciferase reporter. The cell line is validated for the inhibition of the expression of Myc luciferase reporter.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant CHO-K1 cell constitutively expressing a membrane bound, engineered T cell receptor (TCR) activator.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant CHO-K1 cell transfected with empty expression vector containing the hygromycin resistance gene. This cell line serves as a negative control for: TCR Activator - CHO Recombinant Cell line, #60539; DAT CHOK1 Recombinant Cell Line, #60558; and NET - CHOK1 Recombinant Cell , #60557  

Please note this product may be subject to fees, we invite you to contact your local office. The RARβ Reporter (Luc)-HEK293 cell line contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARβ (accession # P10826-2).

Please note this product may be subject to fees, we invite you to contact your local office. The RARγ Reporter (Luc)-HEK293 cell line contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARγ (accession #P13631-1).

Please note this product may be subject to fees, we invite you to contact your local office. The TEAD Reporter - MCF7 cell line contains the firefly luciferase gene under the control of TEAD responsive elements stably integrated into the human breast cancer cell line, MCF7. Inside the cells, basal unphosphorylated YAP/TAZ remains in the nucleus and induces the constitutive expression of luciferase reporter. The cell line is validated for the inhibition of the expression of luciferase reporter by the activators of the Hippo pathway.

Please note this product may be subject to fees, we invite you to contact your local office. The NFAT Reporter - Jurkat Cell Line contains a firefly luciferase gene under the control of the NFAT response element stably integrated into Jurkat cells. This cell line has been validated for response to thapsigargin, ionomycin, and phorbol 12-myristate 13-acetate (PMA). It is useful as a control cell line for other NFAT reporter cell lines expressing various immune checkpoint receptors.

Please note this product may be subject to fees, we invite you to contact your local office. An NF-κB luciferase reporter construct is stably integrated into the genome of CHO-K1 cells. The firefly luciferase gene is controlled by the NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. The NF-κB-luciferase / CHO-K1 cell line is suitable for monitoring the activity of NF-κB transcription factor through luminescence readout.). This cell line responds to human cytokine IL-1β, responds moderately to human TNF?, and does not respond to human IFN-λ (2 µg/ml). Reducing the amount of serum during incubation period may increase the sensitivity to cytokines. Since CHO-K1 cells do not express endogenous human proteins, this cell line provides an excellent platform to enable exogenous expression of a protein of interest to study its

Please note this product may be subject to fees, we invite you to contact your local office. NF-?B luciferase reporter construct is stably integrated into the genome of HCT-116 cells. The<br />firefly luciferase gene is controlled by 4 copies of NF-κ?B response element located upstream of<br />the TATA promoter. Following activation by stimulants, endogenous NF-κ?B transcription factors<br />bind to the DNA response elements to induce transcription of the luciferase gene. The NF-?κB-luciferase/HCT-116 cell line is suitable for monitoring the activity of NF-?κB signaling<br />in response to stimulants such as the cytokines TNF? and IL-1?β, pathogen-associated<br />molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern<br />(DAMP) molecules (i.e. NOD1 ligand) (see application references). It is also suitable for<br />establishing cell-based screens for inhibitors that target specific NF-κ?B stimulating molecules.<br />This cell line can be further mo

Please note this product may be subject to fees, we invite you to contact your local office. The NF-κB reporter (Luc) HEK293 cell line is designed to monitor nuclear factor Kappa B (NF-κB) activity. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or agonists of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. The cell line has been functionally validated in response to human TNF-α, IL-1β, and IL-17.

Please note this product may be subject to fees, we invite you to contact your local office. The Notch CSL Reporter - HEK293 cell line contains the firefly luciferase gene under the control of Notch-response elements (CSL responsive elements) alone with expression construct for Notch1 E (NOTCH1 that has a deletion of the entire extracellular domain) stably integrated into HEK293 cells. Inside the cells, the Notch1 E can be cleaved by γ-secretase. The active Notch1 NICD is released to nucleus and induces the constitutive expression of luciferase reporter. The cell line is validated for the inhibition of the expression of luciferase reporter using a known inhibitor of the Notch signaling pathway.

Please note this product may be subject to fees, we invite you to contact your local office. The SBE Reporter - HEK293 Cell Line is designed for monitoring the activity of the TGF /SMAD signaling pathway. The transforming growth factor beta (TGF ) signaling pathway is involved in a diverse range of cell processes such as differentiation, cell cycle arrest, and immune regulation. TGF signaling has been linked to cardiac disease, cancer, Alzheimer's and other human diseases. TGF proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to phosphorylation and activation of SMAD2 and SMAD3, which then form a complex with SMAD4. The SMAD complex then translocates to the nucleus and binds to the SMAD binding element (SBE) in the nucleus, leading to transcription and expression of TGF / SMAD responsive genes.

Please note this product may be subject to fees, we invite you to contact your local office. The Glucocorticoid Receptor Pathway GAL4 Reporter (Luc) - HEK293 Cell Line contains a firefly luciferase gene under the control of glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR) stably integrated into HEK293 cells. This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. This cell line is validated for response to stimulation of dexamethasone and to the treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway.

Please note this product may be subject to fees, we invite you to contact your local office. The GAL4 Reporter (Luc) - HEK293 Cell Line contains a firefly luciferase gene under the control of a multimerized GAL4 upstream activation sequence (UAS) stably integrated into HEK293 cells. The cell line does not contain any exogenous activators of the GAL4 reporter and can be used alongside BPS Cat. #60655 as a control.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant CHO-K1 cells expressing the cell surface glycoprotein TROP2 (Trophoblast cell-surface antigen 2); ref. seq. NM_002353.2.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant CHO-K1 cells expressing GPC3 (Glypican 3), a cell surface proteoglycan bearing heparin-sulfate; ref. seq. NM_004484.2.

Please note this product may be subject to fees, we invite you to contact your local office. The STAT3 Reporter (eGFP)-HEK293 cell line is designed to monitor the STAT3 signal transduction pathway. It contains an eGFP gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines and growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the eGFP reporter gene.

Please note this product may be subject to fees, we invite you to contact your local office. The NFAT reporter (Luciferase)-THP-1 cell line is designed for monitoring the NFAT (nuclear factor of activated T-cells) signaling pathway in THP-1 cells by measuring luciferase activity. It contains a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter. Upon activation by NFAT activators such as Ionomycin, endogenous NFAT transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant HEK-293 cells expressing enhanced green fluorescent protein (eGFP) under the control of SRE responsive elements. This cell line is validated for its response to EGF or serum stimulation and to treatment with inhibitors of ERK signaling pathway.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal CHO cell line stably expressing full-length human claudin 18.1 (claudin 18 isoform 1, CLDN18, gene accession number NM_016369.4). The stable clonal cell line was selected for high levels of claudin 18.1 expression compared to the parental CHO-K1 cell line.

Please note this product may be subject to fees, we invite you to contact your local office. The STAT3 Reporter Jurkat cell line is designed for monitoring the STAT3 signal transduction pathway. It contains a firefly luciferase gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines or growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

Please note this product may be subject to fees, we invite you to contact your local office. The STAT3 Luciferase Reporter THP-1 cell line is designed for monitoring the STAT3 signal transduction pathway. It contains a firefly luciferase gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines or growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

Please note this product may be subject to fees, we invite you to contact your local office. The Glucocorticoid Receptor (GR)-GAL4 Luciferase Reporter Jurkat Cell Line contains an engineered transcription factor stably integrated into the genome of Jurkat cells, which consists of the glucocorticoid receptor ligand binding domain fused to the DNA binding domain of GAL4. This fusion construct activates firefly luciferase expression which is under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. This cell line is validated for response to stimulation of dexamethasone and to the treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal CHO cell line stably expressing full-length human claudin-6 (CLDN6 gene accession number NM_021195.5).  Surface expression of claudin 6 was confirmed by flow cytometry. The stable clonal cell line was selected for high levels of claudin-6 expression compared to the parental CHO-K1 cell line.

Please note this product may be subject to fees, we invite you to contact your local office. The PD-1 CHO cell line is a recombinant clonal stable CHO-K1 cell line constitutively expressing human PD-1 receptor (accession number: ­­­­­NM_005018). Surface expression of human PD-1 was confirmed by flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal CHO cell line stably expressing full length human claudin 18.2 (claudin18 isoform 2, CLDN18.2, gene accession number NM_001002026.3). The stable clonal cell line was selected for high levels of claudin 18.2 expression compared to the parental CHO-K1 cell line.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant HEK293 cells expressing the firefly luciferase gene under the control of cAMP response element (CRE), and with forced expression of human GIPR (Gastric Inhibitory Polypeptide receptor; NM_000164.4). Activation of GIPR in these cells can be monitored by measuring luciferase activity. The functionality of the GIPR/CRE Luciferase Reporter HEK293 Cell Line was validated in a dose-response assay using agonists gastric inhibitory peptide (GIP) and tirzepatide hydrochloride. These agonists induce luciferase activity in a dose-dependent manner as depicted in Figure 1. <img src="{{media url="wysiwyg/Cell_Lines/78589.jpg"}}" alt="" width="530" height="301" /> Figure 1. Illustration of the GIPR/CRE Luciferase Reporter HEK293 Cell line.

Please note this product may be subject to fees, we invite you to contact your local office. The CRE/CREB Luciferase Reporter CHO Cell Line (cAMP/PKA Signaling Pathway) contains a firefly luciferase gene under the control of multimerized cAMP response element (CRE) stably integrated into CHO cells. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase. This cell line is validated for response to Forskolin.

Please note this product may be subject to fees, we invite you to contact your local office. The NFAT Luciferase Reporter CHO cell line (PKC/Ca2+ Signaling Pathway) contains a firefly luciferase gene under the control of NFAT response element stably integrated into CHO K1 cells. This cell line is validated for its response to phorbol 12-myristate 13-acetate (PMA) in the presence of ionomycin.

Please note this product may be subject to fees, we invite you to contact your local office. The STAT5 Reporter (Luc)-U937 cell line is designed for monitoring STAT5 signal transduction pathway in the U937 cell line. It contains a firefly luciferase gene driven by the STAT5 response element located upstream of the minimal TATA promoter. After activation by GM-CSF, endogenous STAT5 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

Please note this product may be subject to fees, we invite you to contact your local office. The NF-κB reporter (Luc)-Raw 264.7 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal CHO cell line stably expressing full-length human claudin-6 (CLDN6 gene accession number NM_021195.5).  Surface expression of claudin 6 was confirmed by flow cytometry. The stable clonal cell line was selected for high, medium, or low levels of claudin-6 expression compared to the parental CHO-K1 cell line.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal CHO cell line stably expressing full-length human claudin-6 (CLDN6 gene accession number NM_021195.5).  Surface expression of claudin 6 was confirmed by flow cytometry. The stable clonal cell line was selected for high, medium, or low levels of claudin-6 expression compared to the parental CHO-K1 cell line.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal CHO cell line stably expressing full-length human claudin-6 (CLDN6 gene accession number NM_021195.5).  Surface expression of claudin 6 was confirmed by flow cytometry. The stable clonal cell line was selected for high, medium, or low levels of claudin-6 expression compared to the parental CHO-K1 cell line.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal CHO cell line stably expressing full length human claudin 18.2 (claudin 18 isoform 2, CLDN 18.2, gene accession number NM_001002026.3). The stable clonal cell line was selected for different levels of Claudin 18.2 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal CHO cell line stably expressing full length human claudin 18.2 (claudin 18 isoform 2, CLDN 18.2, gene accession number NM_001002026.3). The stable clonal cell line was selected for different levels of Claudin 18.2 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal CHO cell line stably expressing full length human claudin 18.2 (claudin 18 isoform 2, CLDN 18.2, gene accession number NM_001002026.3). The stable clonal cell line was selected for different levels of Claudin 18.2 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line.