Secondary Antibodies

Antibody solution 1 mg/ml in the buffer (0.05M sodium phosphate pH 7.5, 0.1 M NaCl) containing 50% glycerol and 5 mg/ml bovine serum albumin. Human immunoglobulin IgM. No cross-reactivity to human IgG, IgA and other human plasma proteins

The monoclonal antibody 7C2 can be used during various purification steps of IgY. The yolk of eggs laid by immunized chickens has been recognized as an excellent source of polyclonal antibodies (pAb). Specific antibodies produced in chickens offer several important advantages over producing antibodies in other mammals. Because a single egg contains as much antibody as an average bleed from a rabbit, this simple, non-invasive approach presents an appealing alternative to conventional pAb production methods. Purification of chicken egg yolk immunoglobulin Y (IgY), the 150 kDa IgG homolog, does not require animal bleeding. In addition, the eggs from immunized chickens provide a continual, daily source of pAb, and this convenient approach offers greater compatibility with animal protection regulations. Due to the phylogenetic distance between birds and mammals, there is greater potential of producing a higher percentage of specific antibody against mammalian antigens when using chickens. H

The monoclonal antibody 7C2 can be used during various purification steps of IgY. The yolk of eggs laid by immunized chickens has been recognized as an excellent source of polyclonal antibodies (pAb). Specific antibodies produced in chickens offer several important advantages over producing antibodies in other mammals. Because a single egg contains as much antibody as an average bleed from a rabbit, this simple, non-invasive approach presents an appealing alternative to conventional pAb production methods. Purification of chicken egg yolk immunoglobulin Y (IgY), the 150 kDa IgG homolog, does not require animal bleeding. In addition, the eggs from immunized chickens provide a continual, daily source of pAb, and this convenient approach offers greater compatibility with animal protection regulations. Due to the phylogenetic distance between birds and mammals, there is greater potential of producing a higher percentage of specific antibody against mammalian antigens when using chickens. H

Mouse alpha heavy chain of immunoglobulin (determined by immunodot). Avidity on IgA: 4.8 * 109 M-1. Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic) and immunohistochemistry on frozen sections. Available in unconjugated MON5050, HRP conjugated MON5050P, Biotin conjugated MON5050B

Mouse alpha heavy chain of immunoglobulin (determined by immunodot). Avidity on IgA: 4.8 * 109 M-1. Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic) and immunohistochemistry on frozen sections. Available in unconjugated MON5050, HRP conjugated MON5050P, Biotin conjugated MON5050B

Mouse alpha heavy chain of immunoglobulin (determined by immunodot). Avidity on IgA: 2.9 * 109 M-1. Available as unconjugated MON5050, Biotin conjugated MON5050B, Peroxidase conjugated MON5050P.

Mouse epsilon heavy chain of immunoglobulin (determined by immunodot). Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic) and immunohistochemistry on frozen sections. Available in unconjugated MON5051, FITC conjugated MON5051F, HRP conjugated MON5050P and Biotin conjugated MON5051B.

Mouse epsilon heavy chain of immunoglobulin (determined by immunodot). Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic) and immunohistochemistry on frozen sections. Available in unconjugated MON5051, FITC conjugated MON5051F, HRP conjugated MON5050P and Biotin conjugated MON5051B.

Mouse epsilon heavy chain of immunoglobulin (determined by immunodot). Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic) and immunohistochemistry on frozen sections. Available in unconjugated MON5051, FITC conjugated MON5051F, HRP conjugated MON5050P and Biotin conjugated MON5051B.