Secondary Antibodies

Mouse gamma light chain of Immunoglobulin (determined by immunodot) and exhibits no reactivity to mouse lambda light chain. No detectable reactivity with human IgG as tested by ELISA. Available in unconjugated MON5083, FITC conjugated MON5083F, HRP conjugated MON5063H and Biotin conjugated MON5063B.

Mouse Gamma 3 Heavy Chain of Immunoglobulin (determined by immunodot). Avidity on IgG3: 2 * 1010 M-1

The antibody reacts with a Guinea pig lymphocyte subset probably analog to human CD8 (cytotoxic/suppressor) subset. CD8 comprises 2 subunits, alpha and beta and exists as either an alpha/alpha homodimer or an alpha/beta heterodimer. Sequence suggests that guinea pig CD8 is more closely related to human than rat or mouse CD8. Although this monoclonal originally was developed for the detection of a Guinea pig lymphocyte subset, it also can be used as a negative control for the JSB-1 monoclonal antibodies because it is of the same IgG subclass.

Chicken IgY is specific to chickens and is the counterpart to IgG from mammals. Chickens transfer high quantities of IgY into the egg yolk and harvesting antibodies from eggs eliminates the need for the invasive bleeding procedure. One week’s eggs can contain 10 times more antibodies than the volume of rabbit blood obtained from one weekly bleeding. Due to the phylogenetic distance between birds and mammals, there is greater potential of producing a higher percentage of specific antibody against mammalian antigens when using chickens [1]. Since chicken IgY does not cross-react with mammalian IgG [2] and does not bind bacterial or mammalian Fc receptors [3], non-specific binding is reduced, and the need for cross-species immunoabsorptions is also eliminated [4].

Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.

EBS-huA recognizes the third constant domain (CH3) of the alpha chain of human IgA and reacts with both IgA1 and IgA2 isotypes and not with other types of immunoglobulins. IgA type antibodies are secreted by B-cells associated with mucosal epithelia and therefore indicate malignancy if found in lymphoid infiltrates at other locations. Detection of IgA antibodies to EBV in serum may indicate nasopharyngeal carcinoma. Rising IgA anti EBV levels are associated with progression of disease.

SC-05 reacts with a reduction resistant epitope on 80 kDa human secretory component (both free and bound to SIgA). Secretory component is differentially expressed in epithelium, thus SC-05 can identify subpopulations of epithelial cells and epithelial differentiation. Secretory component negative cell lines are not stained with SC-05.

EBS-huG reacts specifically with human IgG and not with other types of immunoglobulins.