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xeno-free hydrogel system for three-dimensional (3D) cultures of mesenchymal stem cells (MSCs). For hydrogel-cell beads for MSC scale-up. No more microcarriers needed. . VitroGel MSC is ready-to-use with an optimized formulation that fully supports the rapid expansion of MSCs. Cells directly thawed from liquid nitrogen or passaged from 2D culture vessels can be immediately mixed with the hydrogel solution for 3D culture or hydrogel-cell bead generation. This hydrogel system is compatible with most MSC culture media and tissue culture vessels. By using the VitroGel Cell Recovery Solution, the cell harvesting after 3D culture is simple and effective.