Cells Reagents

Immediately after delivery, place the passage kit in the dark at -20°C. Prior use thaw the passage kit. After thawing store the passage kit in the dark at 2°C to 8°C for a maximum of 4 weeks. Take care: After thawing shelf time is limited to 4 weeks! After thawing the passage kit can be stored in the dark at 2°C to 8°C for up to 1 month. Do not heat the kit over 37°C or use uncontrollable sources of heat (e.g. microwave appliances). Do not refreeze the kit. This can lead to high salt concentrations by freezing out pure water which will cause irreversible damage. Overtrypsination causes irreversible damage. Because of the tryptic activity of this passage kit, do not exceed the recommended incubation period. Exposing the cells too long will cause irreversible damages to your culture.

Immediately after delivery, place the passage kit in the dark at -20°C. Prior use thaw the tube. After usage store them in the dark at 2°C to 8°C for a maximum of 2 weeks. Take care: After thawing shelf time of Dispase II solution is limited to 14 days! After thawing the passage kit can be stored in the dark at 2°C to 8°C for up to2 weeks. Do not heat the kit over 37°C or use uncontrollable sources of heat (e.g. microwave appliances). Do not refreeze the kit. This can lead to high salt concentrations by freezing out pure water which will cause irreversible damage.

In a serum-free cell culture system, it is very important to cryopreserve cells also in a serum-free freeze medium and not in a serum containing medium. Using an ordinary serum containing freeze medium, the serum affects the cell culture system over a long period. This influence can only be removed by dilution during a large number of passages after seeding. Provitro's Cryo-SFM is a new formulation for cryopreservation of animal and human cells containing no serum, but rather DMSO, methyl cellulose and other cryoprotectant ingredients. After cryopreservation and thawing, a very high percentage of viable cells is obtained. Cells which are cryopreserved in Cryo-SFM also show excellent attachment ability as well as growth performance after thawing. Adherent Cells: Remove adherent cells with Trypsin/EDTA (Provitro Cat.-No.: 204 0001). Neutralize Trypsin with Trypsin inhibitor (Provitro Cat.-No. 204 0001), pellet cells by centrifuging at low acceleration (200 x g) for 3-5 minutes and

Endothelial cell proliferation medium is a sterile liquid culture medium for culturing endothelial cells. The medium is delivered as a basal medium and is suitable for culturing human endothelial cells after adding the supplement mix components. The formulation is optimized for initial seeding of 4,000 cells / cm² up to confluence (approx. 90 %). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. Endothelial cell proliferation medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming efficiency and the population doubling time. Storge: Store the supplemented endothelial c

Microvascular Endothelial Cell Growth Medium, FCS-kit, is a sterile liquid culture medium designed for cultivating human microvascular endothelial cells (HMVEC). Delivered as a basal medium, it is suitable for culturing Provitro HMVEC upon the addition of supplement mix components. The formulation is optimized for the initial seeding of 4,000 cells/cm², allowing growth up to confluence (approximately 90%). The use of feeder layers, matrix substrates, or additional substances is unnecessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. Microvascular endothelial cell proliferation medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming

Smooth Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human smooth muscle cells (HSMC). Provided in the form of a basal medium, it is ideal for culturing HSMC upon the addition of supplement mix components. The formulation is optimized for the initial seeding of 4,000 cells/cm², allowing growth up to confluence (approximately 90%). The use of feeder layers, matrix substrates, or additional substances is unnecessary. To address potential reductions in proliferative activity, we recommend utilizing the antibiotic supplement exclusively for freshly isolated cells. The smooth muscle cell growth medium undergoes comprehensive testing after each production. All components are subjected to rigorous biological assays. Each batch is specifically examined for the proliferating characteristics of human smooth muscle cells (HSMC). The cells cultured in the smooth muscle cell growth medium undergo thorough assessments, including examination of their morpholo

Skeletal Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human skeletal muscle cells (HSKMC). Delivered as a basal medium, it is suitable for culturing Provitro HSKMC upon the addition of supplement mix components. The formulation is optimized for initial seeding of 4,000 cells/cm², facilitating growth up to confluence (approximately 90%). The medium does not require feeder layers, matrix substrates, or additional substances for effective cell cultivation.Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. Skeletal muscle cell growth medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells c

The hMSC Proliferation Medium is a sterile liquid culture medium designed for cultivating human mesenchymal stem cells (hMSC). Delivered as a basal medium, it is well-suited for culturing hMSC upon the addition of supplement mix components. The formulation is optimized for the initial seeding of 6,000 cells/cm², supporting growth up to confluence (approximately 90%). The medium does not require feeder layers, matrix substrates, or additional substances for effective cell cultivation.Due to the possibility of reduced proliferative activity we recommend to use any antibiotic supplement for freshly isolated cells only. Storge: Store the supplemented hMSC proliferation medium in a dark environment at temperatures ranging from 4°C to 8°C for a maximum duration of one month. Avoid heating the medium beyond 37°C and refrain from using uncontrolled heat sources, such

The hMSC osteogenesis induction medium is a sterile liquid culture medium for inducing osteogenic differentiation of human mesenchymal stem cells (hMSC). The medium is delivered as a basal medium and is suitable for culturing hMSC after adding the supplement mix components. The formulation is optimized for initial seeding of 6,000 cells / cm² up to confluence (approx. 90 %). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. After adding 50 ml FCS, 10 ml HEPES and 5 ml L-Glutamine and optional 3,5 ml Antibiotics to the 500 ml hMSC osteogenesis induction medium basal the media can be stored in the dark at 4°C to 8°C for up to 4 weeks. Take care: After adding the Osteogenesis induction factor Dexamethasone, Ascorbic-Acid-2-phosphate and ß-Glycerol-phosphate to the media, the media can be stored in the dark at 4°C to 8°C for up to 1 we