Cell Isolation Tools

Normal Proximal Tubular Cells

Normal Hepatocytes

Normal Alveolar Epithelial Cells II

Normal Pancreatic Epithelial Cells

Normal Thyroid Epithelial Cells

1CellDish is a handheld device with microfluidics aliquoting channels designed for single-cell isolation. The operation is by pipetting cell suspension into the center well and distributing cells to the satellite wells to form single-cell aliquots in less than 2 µL volumes. Features: 1) It provides rapid, convenient, and damage-free cell isolation. 2) It simplifies steps for harvesting target cells and genes. 3) It is compatible with all cell sizes smaller than 50 µm at either low or high cell density. 4) It uniformly distributes cell suspension into 64 outlet wells and retrieves single target cells by pipetting. Parameters: 1) Size of device: standard 60 mm Petri Dish format. 2) Number of wells: 64. 3) Well volume: 2.5 µL. 4) Single-cell yield: about 20 cells per device. Summary: 1CellDish isolates cells at a price below $5 per cell. It is used for single-cell lysis, PCR, sequencing, and multi-omics.

Automated Dissection Chip (AD-Chip) enables yeast cell trapping and culturing. One chip can trap over 10,000 single yeast cells. It allows yeast cells to bud and images are taken to observe the process. As the trapped yeast cells are cultivated and aged with a continuous flow of fresh media, daughter cells that budded from the trapped cells are removed automatically by the media flow throughout the entire lifespan of the trapped mother cells. The AD-Chip, made of biocompatible thermoplastic PMMA, PDMS, and glass, has 75 mm in length and 25 mm in width, corresponding to the standard size of a microscope slide.

1CellAssay® Single-Cell Isolation & Lysis Kit provides a complete solution from easy single-cell isolation to rapid single-cell lysis in a small volume to perform various single-cell analyses, e.g. PCR, sequencing and multi-omics. It contains 1CellDishTM-60mm, 2 devices; Cell Lysis Buffer, 200 µL; 8-strip PCR Tubes, 3 strips. The package size is 8*4*2 inches and the shipping is at room temperature and storage is 4 °C.

1CellAssay® Single-Cell Isolation & 3D Culture Kit provides a complete solution from easy single-cell isolation by 1CellPlate®-96well to efficient 3D cell cloning in hydrogel for generating various spheroids and organoids. It is featuring: 1) Easy operation by regular pipette in a sterile hood; 2) Gentle flow keeps high single-cell viability & integrity; 3) Compatible with cell size ≤ 80 µm and numbers ≤ 100; and 4) Isolate ~ 30 single cells in one 1CellPlate®-96well; 5) No special equipment or operation skills are required. Its applications include: 1) Single-cell isolation from cancer cells, stem cells, CRISPR-edited cells, and primary cells; 2) Single-cell 3D clonal culture in a small volume of xeno-free hydrogel; and 3) Generation of clonal spheroids and organoids for drug toxicity testing and screening. The kit has following components: 1) 1CellPlate®-96well, 5 plates, yielding 30 single cell clones per plate. 2) Pipette inlet adaptor, 5 each, compatible with 20-200 µL pipette to