Cells

The HCMEC/D3 cell line represents an immortalized human cerebral microvascular endothelial cell line, extensively utilized in the study of the blood-brain barrier (BBB). This cell line was generated through the transduction of primary human cerebral microvascular endothelial cells with a lentiviral vector expressing human telomerase reverse transcriptase (hTERT), a crucial enzyme for maintaining telomere length and thereby promoting cellular longevity without transforming the cell phenotype. The introduction of hTERT helps these cells to bypass the replicative senescence that limits the lifespan of primary cells, allowing sustained propagation in culture. HCMEC/D3 cells retain key physiological and morphological characteristics of primary cerebral endothelial cells, making them a valuable model for in vitro studies of the BBB. These include the expression of tight junction proteins such as claudin-5, occludin, and zonula occludens-1, which are critical for maintaining barrier integrit

The HCMEC/D3 cell line represents an immortalized human cerebral microvascular endothelial cell line, extensively utilized in the study of the blood-brain barrier (BBB). This cell line was generated through the transduction of primary human cerebral microvascular endothelial cells with a lentiviral vector expressing human telomerase reverse transcriptase (hTERT), a crucial enzyme for maintaining telomere length and thereby promoting cellular longevity without transforming the cell phenotype. The introduction of hTERT helps these cells to bypass the replicative senescence that limits the lifespan of primary cells, allowing sustained propagation in culture. HCMEC/D3 cells retain key physiological and morphological characteristics of primary cerebral endothelial cells, making them a valuable model for in vitro studies of the BBB. These include the expression of tight junction proteins such as claudin-5, occludin, and zonula occludens-1, which are critical for maintaining barrier integrit

Expanded Human Peripheral Blood NK Cells are NK cells enriched and expanded from human PBMCs using the NK Expansion Kit (BPS Bioscience #78927), a K562 feeder cell-based system for about 2-3 weeks, and cryopreserved. Human Peripheral Blood NK Cells are >90 % pure NK cells (CD3^-CD56⁺ cells), as measured by flow cytometry analysis. They can be used in NK cytotoxicity assays or ADCC (antibody-dependent cellular cytotoxicity), after thaw and recovery in NK Cell Basal Medium for 24 hours or can be further expanded using the BPS Bioscience NK Expansion Kit (BPS Bioscience #78927).

Please note this product may be subject to fees, we invite you to contact your local office.Firefly Luciferase CD20 Knockout Raji Cell Line is a Raji cell line in which CD20 (B-lymphocyte antigen CD20, or MS4A1) has been genetically removed from Raji cells using CRISPR/Cas9 genome editing, while also constitutively expressing firefly (Photinus pyralis) luciferase under the control of a CMV promoter. This cell line was generated by using Firefly Luciferase Lentivirus (BPS Bioscience #79692) on CD20 Knockout Raji Cell Line (BPS Bioscience #82622). This cell line has been validated by genome sequencing, flow cytometry, and luciferase activity measurement.

Please note this product may be subject to fees, we invite you to contact your local office.Firefly Luciferase CD19/CD20 Double Knockout Raji Cell Line is a Raji cell line in which CD19 (Cluster of Differentiation 19, B lymphocyte surface antigen B4, or CVID3) and CD20 (B-lymphocyte antigen CD20, or MS4A1) have been genetically removed from Raji cells using CRISPR/Cas9 genome editing, while also constitutively expressing firefly (Photinus pyralis) luciferase under the control of a CMV promoter. This cell line was generated by using Firefly Luciferase Lentivirus (BPS Bioscience #79692) on CD19/CD20 Double Knockout Raji Cell Line (BPS Bioscience #82623). This cell line has been validated by genome sequencing, flow cytometry and luciferase activity measurement.

Please note this product may be subject to fees, we invite you to contact your local office.BCMA Knockout RPMI-8226 Cell Line is an RPMI-8226 cell line in which BCMA (B-Cell Maturation Antigen, or CD269) has been genetically removed from RPMi-8226 cells using CRISPR/Cas9 genome editing by RNP (ribonucleoprotein) electroporation. This cell line has been validated by genome sequencing and flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office.The DLL4 CHO Cell Line is a CHO-K1 cell line expressing human delta-like 4 (DLL4) gene (accession number NM_019074.4) under the control of the cytomegalovirus (CMV) promoter. This cell line was generated by lentiviral transduction followed by puromycin selection and limiting dilution. Individual clones were screened based on DLL4 expression by flow cytometry to obtain this DLL4 expressing cell line.

Please note this product may be subject to fees, we invite you to contact your local office.BCMA Knockout MM.1S Cell Line is an MM.1S B lymphoblast cell line in which BCMA (B-Cell Maturation Antigen, or CD269) has been genetically removed using CRISPR/Cas9 genome editing. This cell line has been validated by genome sequencing and flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office.PARP1 Knockout MCF7 Cell Line is an MCF7 breast cancer cell line in which human PARP1 (Poly-(ADP-ribose) Polymerase 1) has been genetically removed using CRISPR/Cas9 genome editing. This cell line has been validated by genomic sequencing and Western Blot.