Labelling

The Viability Dye 506 can be used to exclude non-viable cells in flow cytometry assays. It irreversibly binds to cell surface and intracellular amines. The dye can covalently bind to a higher concentration of amines in cells that have lost plasma membrane integrity, such as those in the late stages of apoptosis or dead cells, and generate a significantly more intense fluorescence signal. The advantage of this viability dye over propidium iodide and 7-AAD, is that the cells it denotes can be washed, fixed, permeabilized and intracellularly stained without losing the fluorescence of the viability staining. </p> The Viability Dye 506 can be excited by the violet (405nm) laser and has a peak emission of 506nm detectable by the 510/50 band pass filter.</p> (The 500 tests size will be supplied as 5 x 100 tests.)</p>

The Viability Dye 450 can be used to exclude non-viable cells in flow cytometry assays. It irreversibly binds to cell surface and intracellular amines. The dye can covalently bind to a higher concentration of amines in cells that have lost plasma membrane integrity, such as those in the late stages of apoptosis or dead cells, and generate a significantly more intense fluorescence signal. The advantage of this viability dye over propidium iodide and 7-AAD, is that the cells it denotes can be washed, fixed, permeabilized and intracellularly stained without losing the fluorescence of the viability staining. </p> The Viability Dye 450 can be excited by the violet (405nm) laser and has a peak emission of 450nm detectable by the 450/50 band pass filter.</p> (The 500 tests size will be supplied as 5 x 100 tests.)</p>

The Viability Dye 780 can be used to exclude non-viable cells in flow cytometry assays. It irreversibly binds to cell surface and intracellular amines. The dye can covalently bind to a higher concentration of amines in cells that have lost plasma membrane integrity, such as those in the late stages of apoptosis or dead cells, and generate a significantly more intense fluorescence signal. The advantage of this viability dye over propidium iodide and 7-AAD, is that the cells it denotes can be washed, fixed, permeabilized and intracellularly stained without losing the fluorescence of the viability staining. </p> The Viability Dye 780 can be excited by the red (633nm) laser and has a peak emission of 780nm detectable by the 780/60 band pass filter.</p> (The 500 tests size will be supplied as 5 x 100 tests.)</p>

BP Fluor 488 azide is a popular labeling dye used in Click Chemistry reactions. It will react with the DBCO or other alkyne group in antibody, proteins, peptides, amino-modified oligos, and other target molecules. The dye has an excitation peak at 499 nm and an emission peak at 520 nm. The conjugates are widely used in microscopy, flow cytometry, and other applications. BP Fluor 488 is a pure 5-sulfonated rhodamine molecule and it eliminates the lot-to-lot variation caused by two isomers ratio differences.

BP Fluor 647 Azide is a water-soluble, pH-insensitive from pH 4 to pH 10 bright, far-red fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or BP Fluor 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. BP Fluor 647 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets. For the detection of low abundance targets or where significant increase in signal intensity is desired please consider using next generation azides probes containing an internal copper-chelating motif. Reagent grade, for research purpose. Please con

Rox Passive Reference 300 µl, 5 pack

Rox Passive Reference 300 µl, 10 pack

Rox Passive reference