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Biotin Labeling Kit-NH<sub>2<sub> is primarily used for the preparation of biotin-labeled antibody for enzyme immunoassay (EIA). NH<sub>2<sub>-Reactive Biotin, a component of this kit, has a succinimidyl ester group, and can easily make a covalent bond with an amino group of the target protein or other macromolecules without any activation process.