Labelling

7-(dimethylamino)coumarin-3-carboxylic acid is utilized as a laser dye, fluorescent label, and biomedical inhibitor in various applications.

Dansyl fluoride is a fluorogenic reagent used as a specific fluorescent probe for serine proteases and is a serine-type protease inhibitor. It is an active site directed fluorescent inhibitor of guanidinobenzoatase (GB), which binds to both the colonic isoenzymic forms of the GB. The GB on cell surfaces after binding dansyl fluoride makes the cells fluoresce blue. It has been used for the location of tumour cells in frozen sections of human lung squamous cell carcinoma and colonic carcinoma tissues. It is used as a precursor for the synthesis of other derivatives like dansyl amide. Spectral data: λex 360nm; λem 580nm (methanol).

3,3'-Dipropylthiadicarbocyanine iodide is applied for transmembrane potential measurements.

3,3′-Diethyloxadicarbocyanine iodide is a suitable dye for mode-locking both pulsed and cw tunable rhodamine 6G lasers. It has been used as a micellar probe as it is positively charged and it stays at the micelle–water interface.

Squarylium dye (SQ), which is an analogue of cyanine dye, has a squaraine moiety in the center of the π-conjugate. The SQ dye has a zwitterion structure in which the cation and anion coexist in the molecule, although the cyanine dye has a separated cation component. The SQ was conventionally used as a charge generation material for an organic photo conductors. Recently, SQ provided a sensitizer for organic solar cells originating from a strong absorption in near infrared area. In addition, the SQ dye was applied to an organic light-emitting diode because it shows efficient red luminescence.

7-(Diethylamino)-4-(trifluoromethyl)coumarin is a fluorinated heterocyclic compound with a diethylamine (7-position) and a trifluoromethyl (4-position) substituents. As a coumarin derivative, 7-(Diethylamino)-4-(trifluoromethyl)coumarin is commonly applied as dyes with excellent light conversion capability with quantum yield of 94.8%. It is a promising candidate to be applied in OLEDs and dye-sensitized solar cells (DSSCs). 7-(Diethylamino)-4-(trifluoromethyl)coumarin can be modified with Lawesson's reagent to thiocoumarin. The modification allows further functionalization to the molecules resulting shifts in the absorption and emission spectra. The emission of the coumarin derivatives ranges from 380 nm to 550 nm, which is ideal for bioimaging.

Ethyl 7-(diethylamino)coumarin-3-carboxylate is a reactive molecule that has been used in photochemical reactions to produce molecules with fluorophores. The active methylene group in the molecule reacts with chlorine atoms to form an intermediate that reacts with other intermediate molecules to form the desired product. This compound is not stable and cannot be stored for long periods of time. It can be synthesized from coumarin derivatives, which are compounds derived from aromatic hydrocarbons. Ethyl 7-(diethylamino)coumarin-3-carboxylate has been used as a fluorophore in the synthesis of other compounds due to its fluorescence properties. Hydrogen bonds play an important role in stabilizing this compound and intramolecular hydrogen bonding occurs between the carbonyl oxygen and the amide nitrogen atom. Desulfurization also takes place during synthesis, which makes it more reactive by removing sulfur atoms and

Pyrromethene 567 - used to generate fluorescent conjugates of proteins; nucleotides; oligonucleotides and dextrans; and to prepare fluorescent enzyme substrates; fatty acids; phospholipids; lipopolysaccharides; receptor ligands and polystyrene microspheres.

Stains living cells green. Substrate for esterases. Suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.