Labelling

Di-4-ANEPPS is a voltage-sensitive dye from the Amino-Naphthyl-Ethenyl-Pyridinium (ANEP) family. Initially non-fluorescent, it becomes active when bound to cell membranes and responds to electrical potential changes. This fast-acting dye detects transient (millisecond) potential fluctuations in excitable cells like neurons, cardiac cells, and intact brains. Due to rapid cell internalization, it’s ideal for short-term studies.Di-4-ANEPPS can also serve as a plasma membrane and endocytosis marker.

Di-8-ANEPPS is a potentiometric fluorescent dye that noninvasively measures transmembrane voltage. Initially non-fluorescent, it becomes strongly fluorescent when bound to the lipid bilayer of cell membranes (with an excitation/emission max of approximately 467/631 nm). The fluorescence intensity of Di-8-ANEPPS varies proportionally with changes in transmembrane voltage.

RH 237 is a lipophilic cationic dye used as a fluorescent probe in biological research. It belongs to the rhodamine class of compounds, known for their high brightness and photostability in imaging applications. RH 237 primarily enables functional imaging of neurons, including membrane potential, synaptic activity, and ion channel function. In methanol, RH 237 exhibits an excitation peak at 550 nm and an emission peak at 786 nm. Notably, in cell membranes, the dye’s spectra experience blue shifts of approximately 20 nm for excitation and 80 nm for emission.

AF 488 tyramide is a water-soluble green fluorescent dye used in tyramide signal amplification (TSA) for enhancing fluorescence in immunohistochemistry, immunocytochemistry, and fluorescence in situ hybridization. TSA relies on horseradish peroxidase (HRP) to convert tyramine-containing substrates into highly reactive radicals that covalently bind to nearby proteins. AF 488 tyramide can be used with any HRP-conjugated molecules to stain cells and tissues in immunofluorescence assays.

AF 594 tyramide, a red fluorescent dye, is integral to tyramide signal amplification (TSA), enhancing fluorescence in immunohistochemistry, immunocytochemistry, and fluorescence in situ hybridization (FISH). TSA utilizes horseradish peroxidase (HRP) to convert tyramine-containing substrates into reactive radicals that covalently bind to nearby proteins. AF 594 tyramide can be used with any HRP-conjugated molecules to stain cells and tissues in immunofluorescence assays.

Sulfo-Cy3 tyramide is a water-soluble orange fluorescent dye that is crucial for enhancing fluorescence via tyramide signal amplification (TSA) in immunohistochemistry, cytochemistry, and fluorescence in situ hybridization (FISH). Sulfo-Cy3 tyramide can be used with HRP-conjugated molecules, such as antibodies or streptavidin, for immunofluorescence staining of cells and tissues.

sulfo-Cy5 tyramide, a far-red fluorescent dye, is crucial for enhancing fluorescence via tyramide signal amplification (TSA) in immunohistochemistry, immunocytochemistry, and fluorescence in situ hybridization (FISH). Sulfo-Cy5 tyramide can be used with HRP-conjugated molecules, such as antibodies or streptavidin, for immunofluorescence staining of cells and tissues.