Buffers & Concentrates

Water-soluble tetrazolium salts (WSTs) were developed by introducing positive or negative charges and hydroxy groups to the phenyl ring of the tetrazolium salt. Dojindo’s WSTs have sulfonate groups added directly or indirectly to the phenyl ring to improve water-solubility. Dojindo also offers several newly developed phenylazotype tetrazolium salts, which are easily reduced with NADH or other reducing agents to give orange or purple formazan dyes. Due to the phenylazo group, the color changes with heavy metal ion. Since the water solubility of Dojindo’s WSTs is high, 10 mM to 100 mM solutions can be prepared.

XB-I is a colorimetric reagent for Mg detection. It is slightly soluble in water and alcohol, and readily soluble in aqueous alkaline solution. The aqueous solution of XB-1 is red, and turns reddish-violet in the presence of Mg at pH 9 (maximum wavelength: 510 nm, molar absorptivity: 49,000, detection range 0.02-0.4 ppm).

XB-I is a colorimetric reagent for Mg detection. It is slightly soluble in water and alcohol, and readily soluble in aqueous alkaline solution. The aqueous solution of XB-1 is red, and turns reddish-violet in the presence of Mg at pH 9 (maximum wavelength: 510 nm, molar absorptivity: 49,000, detection range 0.02-0.4 ppm).

XO is a colorimetric reagent and metal indicator of various metal ions. It is readily soluble in water. Its proton dissociation constants are reported to be -1.74, -1.09, 0.76, 1.15, 2.58, 3.23, 6.40, 10.5, and 12.6 (ion strength: 0.2).

XO is a colorimetric reagent and metal indicator of various metal ions. It is readily soluble in water. Its proton dissociation constants are reported to be -1.74, -1.09, 0.76, 1.15, 2.58, 3.23, 6.40, 10.5, and 12.6 (ion strength: 0.2).

Zincon is a colorimetric reagent for Zn and Cu detection, and a metal indicator in EDTA titration.

Zincon is a colorimetric reagent for Zn and Cu detection, and a metal indicator in EDTA titration.

RIPA Lysis Buffer is a complete cell lysis solution reagent used for rapid and efficient total cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells, effectively extracting cytoplasmic, nuclear and membrane proteins. RIPA lysis buffer is highly compatible with various downstream protein analysis applications.

DAB, a widely used chromogen for immunoperoxidase staining, is well accepted among pathologists because of its increased sensitivity and ability to give cleaner background as compared to amino ethylcarbazole (AEC). Specimens stained in DAB can be dehydrated, cleared, and mounted for permanent record keeping. Stable DAB/Plus is more sensitive and stable than traditional working DAB solutions. Peroxidase from the antibody detection system reacts with H2O2 substrate to degrade it, which then reacts with DAB, precipitating it at positive sites yielding a dark brown color.