Buffers & Concentrates

DAB Membrane Peroxidase Substrate (50x) is a benzidine derivative that is oxidized by hydrogen peroxide in the presence of hemoglobin, yielding a dark-brown color of solution. This oxidation can be utilized for tissue samples by first preparing them with hydrogen peroxide. DAB Membrane Peroxidase Substrate (50x) is ideal for researchers in Immunology and Microbiology research.

FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate is an extremely sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP).  FemtoMax™ is designed for both western blotting and enzyme immunoassay (EIA) use.  FemtoMax™ easily allows for the detection of femtogram (10-15) amounts of antigen using photographic film or other imaging methods, including highly sensitive CCD cameras.  Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of detection reagents and re-probed. Use the same blotting conditions for FemtoMax™ as you would when using Amersham ECL Plus™ Substrate or Pierce SuperSignal® West Femto Substrate.

IPTG (isopropyl beta-D-thiogalactoside) Inducer for Beta-Galactosidase Expression acts as a molecular mimic of a lactose metabolite. The presence of IPTG triggers the activation of the lac operon for downstream gene transcription due to its binding the lac repressor. Due to the presence of a sulfur atom in IPTG, the cells cannot degrade the inducer and therefore its concentration remains constant. IPTG (isopropyl beta-D-thiogalactoside) Inducer for Beta-Galactosidase Expression is ideal for investigators in Immunology, Cell Biology, and Microbiology research.

Lysis Buffer is used to lyse cells under nondenaturing conditions. Cell Lysis Buffer is ideal to assist in Immunoprecipitation which allows for the purification by immunoprecipitation of recombinant proteins containing an epitope tag provided by the user. The user is able to further characterize the resultant purified protein by size, post-translational modification, western blot and other assays.

Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.

Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of additional buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. Tris HCl solution (tris(hydroxymethyl)aminomethane hydrochloride) is useful for stabilization of buffer formulations and electrophoresis of biological molecules. Visit our newly expanded web site at www.rockland.com for methods using this and other buffers.

Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.