Buffers & Concentrates

Sodium acetate is the conjugate base of a weak acid. Solution of sodium acetate and acetic acid can act as a buffer to keep a relatively constant pH. This is useful especially in biochemical applications where reactions are pH dependent.

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins. 10X SDS-PAGE Running Buffer is suitable for laboratory involved in protein biochemistry. Visit our newly expanded web site at www.rockland.com for methods using this and other buffers.

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins. Visit our newly expanded web site at www.rockland.com for methods using this and other buffers.

TBE is often used in procedures involving nucleic acids, the most common being electrophoresis in molecular biology. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a chelator of divalent cations, particularly of magnesium (Mg2+). As these ions are necessary co-factors for many enzymes, including contaminant nucleases, the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But since Mg2+ is also a co-factor for many useful DNA-modifying enzymes such as restriction enzymes and DNA polymerases.

TBE is often used in procedures involving nucleic acids, the most common being electrophoresis in molecular biology. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a chelator of divalent cations, particularly of magnesium (Mg2+). As these ions are necessary co-factors for many enzymes, including contaminant nucleases, the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But since Mg2+ is also a co-factor for many useful DNA-modifying enzymes such as restriction enzymes and DNA polymerases.

Tris-Acetate-EDTA (TAE) is a buffer solution containing a mixture of Tris base, acetic acid and EDTA. TAE suitable for multiple molecular biology applications including agarose electrophoresis for the separation of nucleic acids such as DNA and RNA.

Tris-Acetate-EDTA (TAE) is a buffer solution containing a mixture of Tris base, acetic acid and EDTA. TAE suitable for multiple molecular biology applications including agarose electrophoresis for the separation of nucleic acids such as DNA and RNA.

Tris-Phosphate-EDTA (TPE) is a commonly used buffer solution in molecular biology, especially for gel electrophoresis and gene expression studies. The purpose of TPE buffer is to solubilize DNA or RNA, while protecting it from degradation.

Destain Solution is a uniquely formulated, ready-to-use reagent specifically designed for in-gel visualization of Coomassie Blue stained total protein in polyacrylamide gels. The proprietary formulation of the solution ensures high detection sensitivity and low backgrounds.