Buffers & Concentrates

Tris buffered saline with casein is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. It also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.

Revitablot™ Western Blot Stripping Buffer is a uniquely formulated, ready-to-use reagent specifically designed for western blotting. Revitablot™ Western Blot Stripping Buffer is faster and more efficient at stripping of primary and secondary antibodies, including HRP and fluorescent labeled. Blots can be stripped multiple times for the repeated use of membranes. This solution can be used at room temperature. Only incubation of 5-20 minutes needed to strip the membrane. Revitablot™ Western Blot Stripping Buffer is gentle and effective on both nitrocellulose and PVDF western blot membranes.

ProXtract B™ is ready-to-use as a working 1X solution and requires no further dilution. This buffer can be stored and used at room temperature. ProXtract B™ Extraction Buffer is gentle and has a high efficiency of protein recovery. It is compatible with chelating agents, reducing agents, protease inhibitors, cocktail inhibitors and chaotropic salts. There is no interference with downstream purification processes. Other applications may require detergents or other additional components.

ProXtract B™ is ready-to-use as a working 1X solution and requires no further dilution. This buffer can be stored and used at room temperature. ProXtract B™ Extraction Buffer is gentle and has a high efficiency of protein recovery. It is compatible with chelating agents, reducing agents, protease inhibitors, cocktail inhibitors and chaotropic salts. There is no interference with downstream purification processes. Other applications may require detergents or other additional components.

Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) staining buffer is a diluent buffer optimized for use in flow cytometry and immunofluorescence assay of viable and/or fixed cells. FACS and IF staining, washing buffer contains 1% BSA as carrier and stabilizer to reduce non-specific binding of antibodies and fluorochrome reagents to targets cells. Metabolic inhibitors and NaN3 are also included in the flow cytometry and immunofluorescence buffer to prevent patching, capping of surface antigen and bacterial growth.

DNA from Calf Thymus is commonly used in the preparation of prehybridization and hybridization solutions. Ideal for DNA Polymerase Assays. These prepared solutions are used to block the non-specific attachment of probe to the surface of a membrane. The DNA solutions are prepared from highly pure, phenol/chloroform-extracted DNA and DNase-free and RNase-free (DEPC-treated) distilled, deionized water.

DNA from Salmon is commonly used in the preparation of prehybridization and hybridization solutions. These prepared solutions are used to block the non-specific attachment of probe to the surface of a membrane. The DNA solutions are prepared from highly pure, phenol/chloroform-extracted DNA and DNase-free and RNase-free (DEPC-treated) distilled, deionized water.

Lambda phage consists of a virus particle including a head (also known as a capsid), a tail, and tail fibers. The head contains the phage's double-stranded circular DNA genome. The phage particle recognizes and binds to its host, E. coli, causing DNA in the head of the phage to be ejected through the tail into the cytoplasm of the bacterial cell. Lambda phage DNA is commonly used as a DNA molecular weight marker. Digestion of the Lambda phage genome with HindIII produces a set of 8 fragments consisting of 125, 560, 2000, 2300, 4300, 9400, and 23000 base pairs.