Buffers & Concentrates

Ruby Prestained Protein Standard 10-175kDa contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. The standard allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western blot transfer efficiency.

Opal Prestained Protein Standard 10-180kDa is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) made visible when separated on SDS-PAGE (Tris-glycine buffer). Opal Prestained Protein Standard 10-180kDa is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western blot transfer efficiency on membranes (PVDF, nylon , or nitrocellulose) and for approximate sizing of proteins.

Opal Prestained Protein Standard 10-245kDa is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) made visible when separated on SDS-PAGE (Tris-glycine buffer). Opal Prestained Protein Standard 10-245kDa is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western blot transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Opal Prestained Protein Standard 3.5-245kDa is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) made visible when separated on SDS-PAGE (Tris-glycine buffer). Opal Prestained Protein Standard 3.5-245kDa is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western blot transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Sapphire Prestained Protein Standard 10-180kDa is a blue protein standard with 11 pre-stained proteins covering a wide range molecular weights from 10 to180 kDa. Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer). Sapphire Prestained Protein Standard 10-180kDa is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western blot transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Epitope tags are short peptide sequences easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.

Epitope tags are short peptide sequences easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.

BCIP/NBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.

NPP (50X) ELISA ALKALINE PHOSPHATASE SUBSTRATE is a chromogenic substrate utilized in immunosorbent assays. Phosphatase-conjugated detection antibodies can act upon the pNPP to convert it to a yellow para-nitrophenol. This yellow product can be measured on a spectrophotometer at 405nm.