Buffers & Concentrates

Broad Spectrum Protease Inhibitor Cocktail; for inhibiting proteases and esterase.

Boster’s Broad Spectrum Protease Inhibitor Cocktail (EDTA free) is a complex of various protease inhibitors, which has been tested for inhibiting proteases and esterase broadly. 

Boster's ECL Plus Western Blotting Substrate is an ultra-sensitive, luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) at high sensitivity levels (low picogram to mid-femtogram). Boster Western Blotting Substrate may be used for immunoblots, western blots, dot blots and any blotting application utilizing horseradish peroxidase (HRP)-conjugates. The substrate can be used with various blocking buffers and on nitrocellulose or PVDF membranes. Such blots will exhibit low backgrounds. Produced chemiluminescence can be visualized on CCD imaging systems or x-ray film.

10X Detection buffer specially formulated for reducing non-specific binding and maximizing signal-to-background in Phospho and Total THUNDER TR-FRET Cell Signaling Assay Kits. This buffer is already provided in these kits, but can be ordered separately.

Insulin-Transferrin-Selenium-Ethanolamine (ITSE) is a basal media supplement and can be used to reduce the amount of fetal bovine serum (FBS) needed to culture cells. Insulin promotes the uptake of glucose and amino acids, transferrin is a non-toxic iron carrier, selenium is required for the function of glutathione peroxidase and other antioxidant enzymes, and ethanolamine is a phospholipid precursor. The components of the solution are manufactured animal-free and it is provided as a 100x sterile concentrate, 10mL of ITSE concentrate is sufficient for 1 liter of medium.

Insulin-Transferrin-Selenium (ITS) is a basal media supplement and can be used to reduce the amount of fetal bovine serum (FBS) needed to culture cells. Insulin promotes the uptake of glucose and amino acids, transferrin is a non-toxic iron carrier, selenium is required for the function of glutathione peroxidase and other antioxidant enzymes. The components of the solution are manufactured animal-free and it is provided as a 100x sterile concentrate, 10mL of ITS concentrate is sufficient for 1 liter of medium.

CFSE is a reagent useful for cell tracking, proliferation studies, and cell motility studies. After passively diffusing into cells, it is converted to a fluorescent carboxyfluorescin molecule as its acetate groups are cleaved by intracellular esterases. The fluorescent CFSE is retained inside the cell for extended periods, because the molecules forms stable covalent crosslinks with the intracellular proteins. At each cell division, the intensity of the CFSE fluorescence is halved. The peak excitation of the molecule is 494 nm and the peak emission is 521 nm and it may be used with standard fixatives containing formaldehyde and permeabilization buffers that contain saponin.