Proteins & Peptides

1,10-phenanthroline (o-phenanthroline) is a chelator with high affinity for divalent metal ions. When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions. While not specific for DUBs, it has been shown to be a potent inhibitor of JAMM-type isopeptidases through chelation of the active site Zn²⁺ ion. As such, o-phenanthroline is widely used when preparing cell lysates for enrichment of proteins bearing K63-linked polyubiquitin chains which are especially sensitive to DUBs in the JAMM family.

Human PRL 1 (GenBank Accession No. BC023975), a.a. 2-173 with N-terminal His-tag, MW = 19.8 kDa, expressed in an E. coli expression system.

Human PRL 2 (GenBank Accession No. NM_080391), a.a. 2-167 with N-terminal His-tag, MW = 19.1 kDa, expressed in an E. coli expression system.

Human PRL 3 (GenBank Accession No. NM_032611), a.a. 2-173 with N-terminal His-tag, MW = 19.5 kDa, expressed in an E. coli expression system.

Protein tyrosine phosphatase PTP1B, also known as PTPN1 (GenBank accession number M31724), full length, (a.a. 1-436), with N terminal GST-tag, MW=76 kDa, expressed in an E. coli expression system.

Human PTP1B protein tyrosine phosphatase, also known as PTPN1 (GenBank accession no. M31724), a.a. 1-321 (catalytic domain), with N-terminal GST-tag, MW=63.3 kDa, expressed in E. coli.

Rat recombinant PTP1B Protein tyrosine phosphatase, also known as PTPN1 (Genbank accession No. NM_012637.2), a.a. 1-321 (catalytic domain), with N-terminal GST-tag, MW=63.3 kDa, expressed in an E. coli expression system.

Murine recombinant PTP1B Protein tyrosine phosphatase, also known as PTPN1 (Genbank accession No. NM_011201.3), a.a. 1-321 (catalytic domain), with N-terminal GST-tag, MW=63.3 kDa, expressed in an E. coli expression system.