Enzymes

Recombinant human EIF2AK2 (a.a. 252-end), with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Recombinant human EIF2AK3 (a.a. 563-1115), with N-terminal GST-tag, expressed in E. coli cells

Recombinant human GRK2, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Human p70S6Kb1, also known as ribosomal protein S6 kinase, 70 kDa, polypeptide 1 (RPS6KB1), GenBank Accession No. NM_003161, amino acid 1-421 with N-terminal GST tag, MW=74 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human GRK5, also known as GPRK5 (GenBank Accession No. NM_005308), full length, with N-terminal GST tag, MW ~95 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

Human IRAK4 (GenBank Accession No. NM_001114182), a.a. 2-end with N-terminal GST-tag, MW= 77.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human BRAF (b-RAF) 9GenBank Accession #NM_004333), a.a. 417-766 (end) with N-terminal GST-tag, MW = 66 kDa, expressed in Sf9 cells using a Baculovirus expression system.

Human GRK6, also known as GPRK6 or FLJ3213 (GenBank Accession No. BC_017272), full length, with N-terminal GST tag, MW ~93 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

Complex of human LKB1, also known as STK11 (GenBank Accession No. NM_000455, a.a. 2-433, with N-terminal His-tag), MO25 (GenBank Accession No. NM_016289, a.a. 2-341, with N-terminal GST-tag), and STRAD (GenBank Accession No. NM_001003787, a.a. 2-431, with N-terminal GST-tag). Calculated MW=49 kDa* (LKB1), 66 kDa (MO25), and 74 kDa (STRAD), expressed in Sf9 cells using a Baculovirus expression system. *LKB1 runs anomalously on SDS-PAGE.