Enzymes

Recombinant full-length human p38alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is NM_139012.

Mouse JNK1 (GenBank Accession No. NM_016700), full length with N-terminal GST-tag, MW=71 kDa, expressed in a Baculovirus infected Sf9 cell expression system. The mouse amino acid sequence of JNK1 is identical to the one of human JNK1.

Human PAK1 (GenBank Accession No. NM_002576.4), a.a. 248-545, MW=33.6 kDa, expressed in E. coli.

Human PAK2 (GenBank Accession No. NM_002577), a.a. 227-524, MW=33.5 kDa, expressed in E. coli.

Human recombinant LOK1, also known as STK10 (GenBank Accession No. NM_005990), a.a. 1-348 with N-terminal His-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW = 43.2 kDa.

Human MEK1 (K97R) (GenBank Accession No. NM_002755), full length with lysine97 to arginine mutation and C-terminal His-tag, MW=44.3 kDa, expressed in E. coli. The K97R mutation prevents ATP binding, making the kinase inactive.

Human PAK4 (GenBank Accession No. BC002921), a.a. 300-591, MW=33.3 kDa, expressed in E. coli.

Human PAK5 (GenBank Accession No. NM_177990), a.a. 425-719, MW=33.9 kDa, expressed in E. coli.

Human MNK (T385D) (GenBank Accession No. NM_003684), a.a. 2-end with deletion a.a. 165-205 and threonine 385 to aspartic acid mutation, and N-terminal GST-tag, MW=74 kDa, expressed in a Baculovirus infected Sf9 cell expression system.