Enzymes

Human MAPKAPK2 (MK2) (GenBank Accession No. NM_032960), full length with N-terminal GST-tag, MW= 72 kDa, expressed in a baculovirus expression system.

Human MKK6 (GenBank Accession No. NM_002758.3), a.a. 2-end, with N-terminal GST-tag, MW=63.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human MAP3K14 (mitogen-activated protein kinase kinase kinase 14, NIK, GenBank Accession No. NM_003954), a.a. 318-947(end) with N-terminal GST-tag, MW=96 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human MAPK14 (GenBank Accession No. NM_139012), full length, with N-terminal GST-tag, MW=65 kDa, expressed in an E. coli expression system.

Human MAPK10 (mitogen-activated protein kinase 10), also known as PRKM10, JNK3 (c-Jun N-terminal kinase-3), or SAPKβ (stress activated protein kinase-β), GenBank Accession No. NM_002753, a.a. 2-422 (end), with N-terminal GST-tag, MW=75 kDa, expressed in Sf9 cells via a baculovirus expression system.

STK33 (GenBank Accession No. NM_030906.2), a.a. 2-end, with N-terminal GST-tag, MW = 84 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

Complex of recombinant, full length human CDK4 (GenBank accession number NM_000075) with C-terminal His-tag and modifications (a.a. 43G-47G were replaced with EE, and T172A), and human Cyclin D1 (GenBank accession number NM_053056), a.a. 16-271, were co-expressed in a baculovirus-infected Sf9 cells expression system. CDK4 MW=34 kDa, Cyclin D1 MW=29 kDa.

Recombinant full-length human CDK5 (59 kDa) and human p35 (60 kDa), both with N-terminal GST-tags, were co-expressed in Sf9 cells using a baculovirus expression system. The CDK5 gene accession number is NM_004935 and p35 gene accession number is NM_003885.

Complex of recombinant human CDK6, full length with N-terminal His-tag, and Cyclin D1, full length with N-terminal GST-tag (GenBank Accession Nos. NM_001259, and NM_053056 respectively) co-expressed in Sf9 insect cells via a baculovirus expression system.