Enzymes

Complex of recombinant human CDK7, Cyclin H1 and MNAT1, full length, with N-terminal His-tags (CDK7 and MNAT1) and C-terminal His-tag (CyclinH1) , (GenBank Accession Nos. NM_001799, NM_001239, and NM_002431 respectively), co-expressed in Sf9 insect cells via a baculovirus expression system.

Human ULK1 (UNC-51-Like Kinase-1), also known as ATG1 (Autophagy related protein 1), GenBank Accession No. NM_003565, a.a. 2-end with N-terminal FLAG-tag, MW = 113 kDa, expressed in FreeStyle 293-F cells.

Complex of recombinant human CDK1 and CyclinA2, full length, with N-terminal GST-tags (GenBank Accession No. NM_001786, and NM_001237 respectively), co-expressed by Sf9 insect cells via a baculovirus expression system.

Recombinant full-length human CDK2 (GenBank Accession No. NM_001798), a.a. 2-298 (end) with N-terminal GST-tag, MW = 61 kDa, and CyclinA2 (GenBank Accession No. NM_001237), a.a. 174-432 (end) with N-terminal His-GST-tags, MW = 75 kDa. Both proteins were coexpressed in a baculovirus Sf9 insect cell expression system.

Complex of recombinant human CDK2 and CyclinE1, full length, with N-terminal GST-tags (GenBank Accession No. NM_001798, and NM_001238 respectively), co-expressed in Sf9 insect cells via a baculovirus expression system.

Complex of N-terminal GST-tagged recombinant full length human CDK3 (GenBank Accession No. NM_001258), and Cyclin E1 (GenBank Accession No. NM_001238). Co-expressed in a Baculovirus-infected Sf9 cell expression system. CDK3 MW=61 kDa, Cyclin E1 MW=74 kDa.

Complex of recombinant human CDK4 and CyclinD3, full length, with N-terminal GST-tags (GenBank Accession No. NM_000075, and NM_001760 respectively), co-expressed by baculovirus in Sf9 cells

Complex of recombinant full length human CDK5 (GenBank Accession No. NM_004935), a.a. 1-292 (end) with N-terminal GST-tag and p25 (GenBank Accession No. NM_003885), a.a. 99-307 (end) with N-terminal GST-tag. Co-expressed in a Baculovirus-infected Sf9 cell expression system. CDK5 MW=60 kDa, p25 MW=50 kDa.

Complex of recombinant human CDK9 and Cyclin K, full length, with N-terminal GST-tags, (GenBank Accession No. NM_001261, and BC015935, respectively) co-expressed in Sf9 insect cells via a baculovirus expression system.