Enzymes

Human Neuropilin 1 (NRP1), also known as CD304 and VEGF165R, GenBank Accession No. NM_001024628.3, a.a. 22-644(end) with C-terminal Avi-His-tag, expressed in a HEK293 expression system. MW=75 kDa.

Recombinant human Complex of CDK19 and Cyclin. Recombinant human CDK1 (cyclin dependent kinase 12), encompassing amino acids 696-1082. This construct contains an N-terminal GST-tag and includes a Thrombin Cleavage Site to facilitate removal of the GST portion. Recombinant human Cyclin K (CCNK), full length, encompassing amino acids 2-580(end). This construct contains an N-terminal GST-tag and includes a Thrombin Cleavage Site to facilitate removal of the GST portion. These two recombinant proteins were co-expressed and affinity purified.

Recombinant human Elane (Neutrophil Elastase), encompassing amino acids 28-252. The construct contains a C-terminal Avi-Tag™ followed by a His-tag (6xHis). The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration. The protein appears as two separate bands in SDS PAGE due to slightly different glycosylation patterns. The identity of the protein has been confirmed by protein sequencing (by Edman degradation) of the N-terminus. Both bands are recognized by an anti-His antibody, indicating that the C-terminus is intact.

Recombinant human Elane (Neutrophil Elastase), encompassing amino acids 28-252. The construct contains a C-terminal Avi-Tag™ followed by a His-tag (6xHis). The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration. The protein appears as two separate bands in SDS PAGE due to slightly different glycosylation patterns. The identity of the protein has been confirmed by protein sequencing (by Edman degradation) of the N-terminus. Both bands are recognized by an anti-His antibody, indicating that the C-terminus is intact.

Recombinant human Elane (Neutrophil Elastase), encompassing amino acids 28-252. The construct contains a C-terminal Avi-Tag™ followed by a His-tag (6xHis). The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration. The protein appears as two separate bands in SDS PAGE due to slightly different glycosylation patterns. The identity of the protein has been confirmed by protein sequencing (by Edman degradation) of the N-terminus. Both bands are recognized by an anti-His antibody, indicating that the C-terminus is intact.

Recombinant human Elane (Neutrophil Elastase), encompassing amino acids 28-252. The construct contains a C-terminal Avi-Tag™ followed by a His-tag (6xHis). The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration. The protein appears as two separate bands in SDS PAGE due to slightly different glycosylation patterns. The identity of the protein has been confirmed by protein sequencing (by Edman degradation) of the N-terminus. Both bands are recognized by an anti-His antibody, indicating that the C-terminus is intact.

Recombinant human ACE2 (angiotensin converting enzyme 2), encompassing amino acids 18-740. The protein is fused in C-terminal with the Fc fragment of a human IgG1 followed by a C-terminal Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.