Enzymes

Recombinant human PI3 Kinase complex of p110α, with an H1047L mutation, and p85α variant 1. Subunit p110α encompasses amino acids 2-1068(end), and an N-terminal FLAG tag. Subunit p85α encompasses amino acids 1-724(end). The two constructs were expressed together, and the complex was affinity purified.

Recombinant human ALK (C1156Y) (anaplastic lymphoma kinase), encompassing amino acids 1060-end with a C1156Y mutation. This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified and is active.

Recombinant human ALK (F1174L) (anaplastic lymphoma kinase), encompassing amino acids 1060-end with a F1174L mutation. This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified and is active.

Recombinant human ALK (F1174S) (anaplastic lymphoma kinase), encompassing amino acids 1060-end with a F1174S mutation. This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified and is active.

Recombinant human ALK (G1202R) (anaplastic lymphoma kinase), encompassing amino acids 1060-end with a G1202R mutation. This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified and is active.

Recombinant human ALK (G1269A) (anaplastic lymphoma kinase), encompassing amino acids 1060-end with a G1269A mutation. This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified and is active.

Recombinant human ALK (G1269S) (anaplastic lymphoma kinase), encompassing amino acids 1060-end with a G1269S mutation. This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified and is active.

Recombinant human ALK (I1171N, D1203N) (anaplastic lymphoma kinase), encompassing amino acids 1060-end with I1171N and D1203N mutations. This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified and is active.

Recombinant human ALK (L1152R) (anaplastic lymphoma kinase), encompassing amino acids 1060-end with a L1152R mutation. This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified and is active.